This chapter gives an introduction to automated nuclear magnetic resonance (NMR) structure calculation with the program CYANA. Given a sufficiently complete list of assigned chemical shifts and one or several lists of cross-peak positions and columns from two-, three-, or four-dimensional nuclear Overhauser effect spectroscopy (NOESY) spectra, the assignment of the NOESY cross-peaks and the thr...

Nuclear magnetic resonance medical imaging is a new diagnostic tool available to radiologist for investigating the morphology and pathophysiology of the human body. In the presence of constant, stable, and uniform magnetic field and the application of radiofrequency radiation, will enable to produce anatomic cross sections. The physical principles of nuclear magnetic resonance medical imaging a...

Wehave studied nuclear effects on electron spins in lightly doped n-GaAs bulk and quantumwell samples using optically detected magnetic resonance, with the optical detection done via Kerr rotation. The magnetic resonance signal allowed us to measure the electron T ∗ 2 lifetimes and g-factors. We observed strong dynamic nuclear polarization, which was controllable via nuclear magnetic resonance ...

The detailed conformational features and dynamics of the naturally occurring deoxyribotrinucleoside diphosphates d-TpTpT and d-TpTpC have been investigated at 20 degrees C and 80 degrees C in aqueous solution by nuclear magnetic resonance spectroscopy. The observed NMR parameters indicate that the conformational properties of the trimers are very similar to those of the constituent dimers and m...

In this Letter, we present NMR spin-lattice and relaxometry data for proton transfer in one of the shortest known N-H⋯O hydrogen bonds in a single crystal of 3,5 pyridinedicarboxylic acid (35PDCA). It is widely believed that proton transfer by quantum tunneling does not occur in short hydrogen bonds since the ground state energy level lies above the potential barrier, yet these data show a temp...

The rheology is widely known as one of the basic methods for rubber investigations for many years. At the same time, a variety of other methods of physical and chemical analysis suitable is available for rubber industry. One of them, the Time-Domain Nuclear Magnetic Resonance (TD--NMR) Spectroscopy is very sensitive to structural properties of elastomer materials. Moreover, by using NMR it is p...

background hepatic fibrosis is a typical complication of chronic liver diseases resulting in cirrhosis that remains a major public health problem worldwide. liver biopsy is currently the gold standard for diagnosing and staging hepatic fibrosis. percutaneous liver biopsy; however, is an invasive procedure with risks of complications. therefore, there is need for alternative non-invasive techniq...

Myriad biological processes proceed through states that defy characterization by conventional atomic-resolution structural biological methods. The invisibility of these 'dark' states can arise from their transient nature, low equilibrium population, large molecular weight, and/or heterogeneity. Although they are invisible, these dark states underlie a range of processes, acting as encounter com...

In the Spring of 2013, NMR spectroscopists convened at the Weizmann Institute in Israel to brainstorm on approaches to improve the sensitivity of NMR experiments, particularly when applied in biomolecular settings. This multi-author interdisciplinary Review presents a state-of-the-art description of the primary approaches that were considered. Topics discussed included the future of ultrahigh-f...

Classical structural biology approaches allow structural characterization of biological macromolecules in vitro, far from their physiological context. Nowadays, thanks to the wealth of structural data available and to technological and methodological advances, the interest of the research community is gradually shifting from pure structural determination towards the study of functional aspects ...