The paper describes an improved image analysis procedure for measuring the DNA content of cell nuclei in thick sections of liver tissue by absorption densitometry. Whereas previous methods only permitted the analysis of isolated nuclei, the new technique enables both isolated and overlapping nuclei to be measured. A 3D segmentation procedure determines whether each object is an isolated nucleus...

Live cell microarray technology [1], allows the simultaneous analysis of many gene products. These microarrays are growing cells in spots printed on a glass slide using a robotic arrayer. The cells growing on the DNA and gelatin spots express the DNA and divide 2–3 times in the process of creating a microarray with features consisting of clusters of transfected cells. High-resolution images are...

A new method for segmenting images of immunohistochemically stained cell nuclei is presented. The aim is to distinguish between cell nuclei with a positive staining reaction and other cell nuclei, and to make it possible to quantify the reaction. First, a new supervised algorithm for creating a pixel classifier is applied to an image that is typical for the sample. The training phase of the cla...