The objective of this study was to evaluate the effects of metal ions, which may be released from orthopedic or dental implants, on osteoblast metabolism and differentiation. ROS 17/2.8 cells were cultured in F-12 medium for 7 days. Then Al+3, Co+2, Cr+3, Ni+2, Ti+4, and V+3 were added at concentrations less than their cytotoxic concentrations. After 3 days, DNA synthesis, succinate dehydrogena...

Phenotyping of Gprc6a KO mice has shown that this promiscuous class C G protein coupled receptor is variously involved in regulation of metabolism, inflammation and endocrine function. Such effects are described as mediated by extracellular calcium, L-amino acids, the bone-derived peptide osteocalcin (OCN) and the male hormone testosterone, introducing the concept of a bone-energy-metabolism-re...

The failure of an osseous fracture to heal (development of a non-union) is a common and debilitating clinical problem. Mice lacking the tumor suppressor Pten in osteoblasts have dramatic and progressive increases in bone volume and density throughout life. Since fracture healing is a recapitulation of bone development, we investigated the process of fracture healing in mice lacking Pten in oste...

PURPOSE/OBJECTIVES To conduct a role delineation study of basic oncology nursing practice as a basis for revision of the blueprint for the Oncology Certified Nurse (OCN) examination. DESIGN Three-phase study of oncology nurses' practice. SAMPLE 735 oncology nurses randomly chosen from all nurses who are OCN certified. METHODS A pilot survey was mailed to a small group to allow refinement ...

Calcific aortic valve stenosis (CAVS) is a heart disease characterized by the progressive fibro-calcific remodeling of valves, an actively regulated process with involvement reactive oxygen species-mediated differentiation valvular interstitial cells (VICs) into osteoblast-like cells. Nuclear factor erythroid 2-related 2 (Nrf2) regulates expression variety antioxidant genes, and plays protectiv...

Precise information on the effects of extracorporeal shockwave therapy (ESWT) biochemical status in humans is still lacking. Hence, this study investigated effectiveness three or six sessions low energy ESWT serum bone metabolism markers individuals who have undergone post anterior cruciate ligament (ACL) reconstruction. Participants with ACL injuries were assigned into groups (n=10 per group),...

The problem is the locally finite generation of a relation sheaf R(τ1, . . . , τq) in OCn . After τj reduced to Weierstrass’ polynomials in zn, it is the key for applying an induction on n to show that elements of R(τ1, . . . , τq) are expressed as a finite linear sum of zn-polynomiallike elements of degree at most p = maxj degzn τj over OCn . In that proof one is used to use a division by τj o...

Octachloronaphthalene (OCN), a serious environmental pollutant, has been investigated by charge density analysis to unravel several unexplored factors responsible for steric overcrowding. The topological features of the enigmatic peri interactions contributing to steric overcrowding are qualified and quantified from experimental and theoretical charge-density studies. A new facet in the fundame...

Production of the cells that ultimately populate the thymus to generate α/β T cells has been controversial, and their molecular drivers remain undefined. Here, we report that specific deletion of bone-producing osteocalcin (Ocn)-expressing cells in vivo markedly reduces T-competent progenitors and thymus-homing receptor expression among bone marrow hematopoietic cells. Decreased intrathymic T c...

A simple interface based on an oscillating capillary nebulizer (OCN) is described for direct deposition of eluate from a thermal field-flow fractionation (ThFFF) system onto a matrix-assisted laser desorption/ionization (MALDI) probe. In this study, the polymer-containing eluent from the ThFFF system was mixed on-line with MALDI matrix solution and deposited directly onto a moving MALDI probe. ...