Monoclonal antibodies against rabbit ocular mucin (ROM) and porcine stomach mucin (PSM) were developed to explore the biosynthesis and functional significance of ocular mucin. A nitrocellulose-based dot enzyme-linked immunosorbent assay (ELISA) was developed for hybridoma screening and mucin quantitation. The sensitivity was found to be at the level of 1 ng mucin per dot, which was about 1000 t...

PURPOSE To describe the clinical spectrum of lesions involving the visual system in von Hippel-Lindau (VHL) disease, the genetic alterations, and the molecular genetic properties of retinal hemangioblastomas. METHODS In this prospective case-series, 406 patients with VHL disease had systemic and ocular evaluations. Genetic mutations within six pathological specimens were evaluated, using micr...

PURPOSE There is evidence from theoretical models and animal studies that the biomechanical properties of the optic nerve head and the sclera play a role in the pathophysiology of glaucoma. There are, however, only a few data available that demonstrate such biomechanical alterations in vivo. In this study, the hypothesis was that patients with primary open-angle glaucoma (POAG) have an abnormal...

ABSTRACT: To determine the association between ocular abnormalities and hematologic findings in Ehrlichia canis-infected dogs, 120 dogs suspected of canine ehrlichiosis were studied. All patients subjected to rapid serologic diagnostic test for canis, diagnosis was confirmed by quantitative PCR. PCR-positive underwent ophthalmologic examination a hemogram grouped into with thrombocytopenia anem...

Purpose: To study the frequency and characteristics of ocular manifestations in patients with multiple sclerosis (MS) in the state of São Paulo. Methods: From March, 1996 to November, 1998, 64 patients (48 females, 16 males, age 17 59 years) with MS underwent ophthalmologic examination and computerized visual field perimeter testing. Results: Forty-four (68.75%) of the 64 examined patients pres...

The existence of the ocular microbiota has been reported but functional analyses to evaluate its significance in regulating ocular immunity are currently lacking. We compared the relative contribution of eye and gut commensals in regulating the ocular susceptibility to Pseudomonas aeruginosa-induced keratitis. We find that in health, the presence of microbiota strengthened the ocular innate imm...

PURPOSE OF REVIEW Hyperosmolarity is a central mechanism causing ocular surface inflammation and eye irritation in typical patients suffering from tear dysfunction. Tear composition in dry eyes, or dysfunctional tear syndrome, may destabilize the tear film and cause ocular surface epithelial disease. Increased activity of matrix metalloproteinases (MMPs), especially MMP-9, plays a critical role...

Purpose A growing body of evidence suggests that the microbiome of the ocular surface confers potent immunoregulatory functions and has a key role in the physiologic maintenance of healthy eyes and in the pathogenesis of ocular diseases. Although the microbiome is known to be affected by age and sex, the influence of these factors on ocular surface microbiota in healthy adults remains largely u...

Diabetic retinopathy (DR) is a microvascular lesion that occurs as complication of diabetes mellitus. Many studies reveal retinal neurodegeneration early in its pathogenesis, and abnormal function can occur patients without any signs abnormalities. The gut microbiota large, diverse colony microorganisms colonize the human intestine. Studies indicated involved pathophysiological processes DR pla...