In this work, the biodegradation of the crude oil by the potential biosurfactant producing Bacillus subtilis A1 was investigated. The isolate had the ability to synthesize degradative enzymes such as alkane hydroxylase and alcohol dehydrogenase at the time of biodegradation of hydrocarbon. The biosurfactant producing conditions were optimized as pH 7.0, temperature 40°C, 2% sucrose and 3% of ye...

The use of an organic solvent can allow to increase the mass transfer, and thereby biodegradation, of hydrophobic gaseous pollutants. The biodegradation of hexane by a Pseudomonas aeruginosa strain was thus optimized in a two phase partitioning bioreactor (TPPB). Silicone oil was first selected among various organic solvents based on its biocompatibility and resistance to P. aeruginosa and its ...

The aim of this work was to study the biodegradation of different types of automotive lubricant oils adapted to the aqueous medium using a base inoculum and an aqueous inoculum. Four treatments were carried out in two consecutive and similar experiments: T1 (control); T2 (half-synthetic oil); T3 (mineral oil); T4 (used oil). The results showed the following decreasing order of CO2 production in...

Crude oil extraction, transportation and use provoke the contamination of countless ecosystems. Therefore, bioremediation through surfactants mobilization or biodegradation is an important subject, both economically and environmentally. Bioremediation research had a great boost with the recent advances in Metagenomics, as it enabled the sequencing of uncultured microorganisms providing new insi...

Nutrient amendment to oil-contaminated beach sediments is a critical factor for the enhancement of indigenous microbial activity and biodegradation of petroleum hydrocarbons in the intertidal marine environment. In this study, we investigated the stimulatory effect of the slow-release fertilizers Osmocote (Os; Scotts, Marysville, OH) and Inipol EAP-22 (Ip; ATOFINA Chemicals, Philadelphia, PA) c...

Although oil-based drilling fluids are of paramount practical and economical interest, they represent a serious source of pollution, once released into the environment as drill cuttings. The aim of this study is to assess the capability of isolated microorganisms to degrade gasoil fuel. The commonly used physicochemical and biodegradation remediation techniques of petroleum contaminated soil we...

EXECUTIVE SUMMARY The objective of this document is to present a detailed technical guidance document for use by spill responders for the bioremediation of marine shorelines and freshwater wetlands contaminated with oil and oil products. Technical personnel who are responsible for designing and operating field bioremediation processes as well as consultants and equipment manufacturers will also...

A pipeline transporting crude-oil broke in a nature reserve in 2009 and spilled 5100 m(3) of oil that partly reached the aquifer and formed progressively a floating oil lens. Groundwater monitoring started immediately after the spill and crude-oil recovery by dual pump-and-skim technology was operated after oil lens formation. This study aimed at documenting the implementation of redox-specific...

OBJECTIVE To biodegrade the diesel pollution in aqueous solution inoculated with Mycobacterium and filamentous fungi. METHODS Bacteria sampled from petroleum hydrocarbons contaminated sites in Karamay Oilfield were isolated and identified as Mycobacterium hyalinum (MH) and cladosporium. Spectrophotometry and gas chromatography (GC) were used to analyze of the residual concentrations of diesel...