BACKGROUND Surplus oocytes in assisted reproduction treatment cycles could be saved and donated to other couples. ICSI is usually performed for oocytes that have been stored frozen, considering possible exocytosis of cortical granules (CG). The unavoidability of ICSI merits further study. METHODS We used a slow method to freeze excess oocytes from infertile couples. After thawing, oocytes wer...

The present study examined the kinetics of glutathione (GSH) concentration during maturation and after fertilization in pig oocytes and its relevance to the ability of pig oocytes to form a male pronucleus after in vitro fertilization. The GSH concentration was significantly higher in pig oocytes matured in Waymouth medium than in pig oocytes matured in either modified (m) TCM-199 or mTLP media...

Pig oocytes were hand collected after follicular puncture or were mass harvested with stainless-steel screens and purified on sucrose density gradients. Most oocytes obtained by mass harvesting with a 75 micron screen and selecting on a 0 and 30% discontinuous sucrose gradient were meiotically incompetent and atretic after culture. Two enriched populations of oocytes were isolated from the 0-15...

OBJECTIVE Autophagy contributes to the clearance and recycling of macromolecules and organelles in response to stress. We previously reported that vitrified mouse oocytes show acute increases in autophagy during warming. Herein, we investigate the potential role of Atg7 in oocyte vitrification by using an oocyte-specific deletion model of the Atg7 gene, a crucial upstream gene in the autophagic...

Cyclic nucleotides cAMP and cGMP are among the main molecules that control maturation of mammal oocytes. The in vitro simulated physiological oocyte (SPOM) method was used to model accumulation oocyte. In current study, we preincubated oocytes cows (not primed with FSH) modulators: N 6,2’-O-dibutyryladenosine 3’,5’-cyclomonophosphate (dbcAMP) 3-isobutyl-1-methylxanthine (IBMX). use SPOM increas...

introduction: the purpose of this study was to evaluate the effect of different doses of retinoic acid on resumption of meiosis, in vitro maturation of oocytes and resulting embryo development in mice. materials and methods: germinal vesicle of nmri female mouse (6-8 weeks old) oocytes were collected from ovaries and cultured in maturation medium mem-α contained: 100 mliu/ml rfsh + 7.5 iu/ml hc...

There is currently much interest in assisted reproduction techniques in the horse, however, many aspects of oocyte maturation, fertilization, and embryo development in the horse differ from those in other species. Because of the close attachment of the equine oocyte to the follicle wall, scraping of the follicle is the most effective method for oocyte recovery. A notable feature of equine oocyt...

Niemann-Pick C1 Like 1 (NPC1L1) is known to be involved in the intestinal absorption of cholesterol. For evaluating function NPC1L1, cell lines such as Caco-2, Madin-Darby canine kidney (MDCK) II, and McA-RH7777 have been used previous studies, but detailed molecular mechanism transport has not elucidated. In this study, characteristics cholesterol via NPC1L1 were investigated using a Xenopus l...

Rat oocytes can be produced artificially by superovulation. Because some strains show low sensitivity to superovulation treatment, in vitro maturation is an alternative method to produce numerous matured oocytes. Furthermore, establishment of an in vitro maturation system with simple culture conditions is cost effective and leads to easy handling of oocytes. This study examined developmental ab...

The aim of the present study was to examine the fertilization ability and mitochondrial function of oocytes derived from cows with or without liver damage. Oocytes were collected from the ovaries of cows with damaged livers (DL) and those of cows with healthy livers (HL), subjected to in vitro maturation, and fertilized in vitro. A significantly high abnormal fertilization rate was observed for...