We have constructed an integrated optical microscope that allows for the simultaneous image acquisition from multiple optical imaging modalities. The microscope consists primarily of hardware for spectral-domain optical coherence microscopy (OCM), multi-photon microscopy (MPM), and second harmonic generation microscopy. The unique configuration of the integrated microscope allows for the acquis...

This paper reports a high-throughput, label-free technique to visualize individual carbon nanotubes (CNTs) on a silicon wafer using a conventional optical microscope. We show that individual CNTs can locally enhance the rate of vapor-phase HF etching of SiO2 to produce a SiO2 trench that is several to several tens of nanometers in depth. The trench is visible under an optical microscope due to ...

The resolution of an optical microscope is restricted by the diffraction limit, which is approximately 200 nm for a white light source. We report that sub-diffraction-limited objects can be resolved in immersion liquids using a microsphere optical nanoscopy (MONS) technique. Image magnifications and resolutions were obtained experimentally and compared in different immersion liquids. We show th...

We present a compact laser scanning two-photon excitation (TPE) microscope for randomaccess imaging based on a low-cost digital micromirror device (DMD) that scans the laser beam in X, Y and Z axes at the DMD pattern rate, i.e. 4.2 ~32.5 kHz. The optical configuration of the TPE microscope is presented in Figure 1. The X, Y, Z scanning is achieved by programming binary hologram patterns [1] to ...

The purpose of the report is to describe the state of the optical microscope parts market in the Iberian countries, to present actual and retrospective information about the volumes, dynamics, structure and characteristics of production, imports, exports and consumption and to build a forecast for the market in the next five years. In addition, the report presents an elaborate analysis of the m...

In this Letter, we demonstrate the experimental mapping of the longitudinal magnetic and electric optical fields with a standard scanning microscope that involves a high-numerical-aperture far-field objective. The imaging concept relies upon the insertion of an azimuthal or a radial polarizer within the detection path of the microscope that acts as an optical electromagnetic filter aimed at tra...

This paper presents micropositioning system using feedback of multiple magnification images from CCD cameras mounted on an optical microscope. In order to guarantee of the accurate micropositioning to micro assembly system, high precise sensors are required. But many kinds of sensors, which are used in macro world, have insufficient resolution and size. The visual data from an optical microscop...

Two-photon fluorescence microscopy provides a powerful tool for deep tissue imaging. However, optical aberrations from illumination beam path limit imaging depth and resolution. Adaptive Optics (AO) is found to be useful to compensate for optical aberrations and improve image resolution and contrast from two-photon excitation. We have developed an AO system relying on a MEMS Deformable Mirror (...

We demonstrate quantitative phase mapping in confocal optical microscopy by applying synthetic optical holography (SOH), a recently introduced method for technically simple and fast phase imaging in scanning optical microscopy. SOH is implemented in a confocal microscope by simply adding a linearly moving reference mirror to the microscope setup, which generates a synthetic reference wave analo...

The term used in a title is rather uncertain. In microscopy the recent progress of optical methods resulted in the appearance of a large number of techniques many of which can be considered as classical. However, the purpose of this chapter is not to review the wide capabilities of optical microscopy but define some terms which will be used further for the explanation of a confocal microscope p...