background: culture media enrichment through the addition of protein hydrolysates is beneficial for achieving higher protein expression. methods: in this study, designing the optimum mixture of four soy and casein-derived hydrolysates was successfully performed by design of experiment and specific productivity increased in all predicted combinations. protein profile of recombinant cho (rcho) ce...

background the development of an effective subunit vaccine against brucellosis is a research area of intense. but optimization of recombinant proteins production in escherichia coli and content of endotoxins associated with final recombinant proteins are very important. objectives in the present study, expression and purification of brucella melitensis rhsp and rtf were optimized to reduce endo...

Background: Interferons are some kind of natural cytokines which express in response to a variety of antigens including viral RNA, bacterial products, and tumor proteins. Interferon beta is used in the treatment of autoimmune diseases such as multiple sclerosis. Moreover, this drug inhibits cellular proliferation as well as angiogenesis and as a result, helps to cure cancer. In this research, i...

abstract: regarding the growing use of distributed generation resources, optimal design of these units is necessary in the power system especially in the distribution network. in this paper, cuckoo optimization algorithm with multiple purposes was used to determine optimal location and size of distributed generation resources. the investigated purposes include reducing line losses, reducing med...

human epidermal growth factor receptor (her) family plays an important role in various types of cancers. as a result, antibodies against her and the mechanism of antigen-antibody binding action are under active investigation. we previously constructed a single-chain variable fragment (scfv) against her2, i.e. anti-her2 scfv, for expressing in the escherichia coli . in the present study, we repo...

Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...