Isothermal titration calorimetry (ITC) may be used to determine the kinetic parameters of enzyme-catalyzed reactions when neither products nor reactants are spectrophotometrically visible and when the reaction products are unknown. We report here the use of the multiple injection method of ITC to characterize the catalytic properties of oxalate oxidase (OxOx) from Ceriporiopsis subvermispora (C...

In this simple, sensitive, and rapid enzymatic method for the determination of oxalate in urine or plasma, oxalate oxidase (EC 1.2.3.4) prepared from barley seedlings is used to convert oxalate to carbon dioxide and hydrogen peroxide, which is determined photometrically at 600 nm, with use of horseradish peroxidase, by oxidative coupling of 3-methyl-2-benzothiazoline hydrazine with N,N-dimethyl...

High-throughput transcriptomics was used to identify Fibroporia radiculosa genes that were differentially regulated during colonization of wood treated with a copper-based preservative. The transcriptome was profiled at two time points while the fungus was growing on wood treated with micronized copper quat (MCQ). A total of 917 transcripts were differentially expressed. Fifty-eight of these ge...

Ceriporiopsis subvermispora oxalate oxidase (CsOxOx) is the first bicupin enzyme identified that catalyzes manganese-dependent oxidation of oxalate. In previous work, we have shown that the dominant contribution to catalysis comes from the monoprotonated form of oxalate binding to a form of the enzyme in which an active site carboxylic acid residue must be unprotonated. CsOxOx shares greatest s...

The Oxalate Salt of P-Aminodimethylaniline, an Improved Reagent for the Oxidase Test: Charles M. Carpenter, Leif G. Suhrland, and Martha Morrison ............................... 649 Histochemical Method for the Detection of Phosphorylase in Plant Tissues: H. C. Yin and C. N. Sun 650 Use of Aerosol OT in Dissecting Salivary Glands of Mosquitoes Infected With Malaria: Edward S. Josephson, William...

We report an analytical system in which glucose oxidase immobilized on controlled-pore glass is used. The immobilized glucose oxidase was packed in a column that was part of a continuous-flow system. Oxygen depletion of the buffer was measured with a Clark-type electrode, and the resulting data were stored on tape and then evaluated with a PDP-12 computer. The relative advantages of several mea...

CURRENT SCIENCE, VOL. 81, NO. 3, 10 AUGUST 2001 248 by repeating the experiment at least once. A total of 34 decamer primers (series OPA, OPB etc.) from Operon Tech, CA, USA were screened using three representative genomic DNA samples of black pepper. Of these, 24 primers that yielded consistent and clear band patterns were selected for the final analysis of the 22 accessions used in the presen...