The concentrations of ROS were measured in samples of the sensorimotor brain cortex and in the rat blood. We measured the following parameters: The six lines spectra, nitroxide radical, free hydroxyl radical and singleton oxygen. Their concentration was measured under physiological conditions, after the nociceptive stimulation and after the application of melatonin, both in normal and stimulate...

Direct electron spin resonance was used to detect tert-alkylperoxyl radicals generated by hematin and the corresponding hydroperoxides at near-physiological pH values. The spin-trapping method was necessary to detect the less persistent primary ethylperoxyl radical. Under a nitrogen atmosphere, the electron spin resonance signal of the tert-alkylperoxyl radicals decreased, and the ethylperoxyl ...

the first reaction between food components and biological tissues occurs in the oral cavity. saliva modulates the ecosystem in the oral cavity and plays a critical role in oral homeostasis. in addition, saliva is a first line of defense against free radical-mediated stress. salivary antioxidant defense mechanisms seem to be very important. in this study we discuss in detail free radicals (fr) a...

musa sapientum (m.sapientum) commonly known as ‘banana’ is widely used in bangladeshi folk medicine for the treatment of various ailments including diarrhea. hence, the present study was designed to investigate antidiarrheal, antioxidant and antibacterial potential of the methanolic extract of m.sapientum seed (mmss). the extract was studied for antidiarrheal property using castor oil and magne...

introduction: free radicals are molecules or atoms with single electron that results in reactions thus causing severe damages to the body’s macromolecules. by preventing the formation of free radicals, repairing damages caused by radical’s activities, increasing the excretion of the damaged molecules and minimizing the cellular mutation, the antioxidant defense system contrasts the damages caus...

Mitomycin C (MC) is a naturally occurring anticancer agent which has been shownto be more cytotoxicto hypoxictumor cells than to their aerobic counterparts. The mechanism of action of this agent is thought to involvebiologicalreductiveactivation,to a speciesthat alkylutes DNA. A comparisonof the cytotoxicityof MC to EMT6 tumor cells with that of the structural analogues porfiromycin(PM), .•V-...

The realization that reactive oxygen species and oxidative stress play an important role in the etiology and progression of major human degenerative diseases has triggered enormous and worldwide interest in endogenous and exogenous antioxidants. The 1990’s have seen the explosion of interest in antioxidants. The article reviews free radical chemistry, antioxidant systems & their role in human d...

Oxygen free radicals are highly reactive species generated by many cellular oxidation-reduction processes. These radicals damage cellular constituents and have been causally implicated in the pathogenesis of many human diseases. We report here that oxygen free radicals generated by Fe2+ in aqueous solution are mutagenic. Aerobic incubation of luminal diameter X174 am3 (amber 3 mutation) DNA wit...

Alcohol treatment results in increases in the release of endotoxin from gut bacteria and membrane permeability of the gut to endotoxin, or both. Females are more sensitive to these changes. Elevated levels of endotoxin activate Kupffer cells to release substances such as eicosanoids, TNF-alpha and free radicals. Prostaglandins increase oxygen uptake and most likely are responsible for the hyper...

The dietary carotenoids provide photoprotection to photosynthetic organisms, the eye and the skin. The protection mechanisms involve both quenching of singlet oxygen and of damaging free radicals. The mechanisms for singlet oxygen quenching and protection against free radicals are quite different - indeed, under some conditions, quenching of free radicals can lead to a switch from a beneficial ...