An isothermal amplification method was developed for the sensitive detection of the H5N1 influenza virus. The padlock probe specifically bound to the H5N1 target and circularized with T4 DNA ligase enzyme. Then this circular probe was amplified by hyperbranched rolling circle amplification (HRCA) using Phi29 DNA polymerase. The fluorescence intensity was recorded at different intervals by inter...

conclusions species identification of trichophyton is crucially important for epidemiological and phylogenetic purposes and for genotype delineation. rca based on its polymorphisms can be used to generate identification barcodes and as an alternative to dna sequencing; it is a very fast, specific, and economical tool for species identification. results of the 61 putative trichophyton clinical i...

We study whether the padlock and the signal strength bars, two visual cues shown in network managers, convey their intended messages. Since users often choose insecure networks when they should not, finding the answer is not obvious; in our study we clarify whether the problem lies in uninformative and ambiguous cues or in the user who, despite understanding the cues, chooses otherwise. This pa...

A 27-year-old woman with severe neurological disability and portal hypertension secondary to Wilson’s disease was referred for endoscopic closure of a chronic gastrocutaneous fistula following the removal of a percutaneous endoscopic gastrostomy (PEG) tube 8 months previously (●" Fig.1). The patient suffered from skin denudation caused by leakage of gastric secretions. At upper gastrointestinal...

Ligation-based nucleic acid detection methods are primarily limited to DNA, since they exhibit poor performance on RNA. This is attributed to reduced end-joining efficiency and/or fidelity of ligases. Interestingly, chlorella virus DNA ligase (PBCV-1 DNA ligase) has recently been shown to possess high RNA-templated DNA end-joining activity; however, its fidelity has not yet been systematically ...

We describe our experience with a new over-the-scope clip (OTSC) system (Padlock Clip?) in the treatment of 14 patients. Eight of the 14 patients were treated for closure of gastrointestinal fistulas (n?=?4), iatrogenic gastrointestinal perforations (n?=?2), or hemostasis of post-polypectomy bleeding (n?=?2). The site of clipping was the lower gastrointestinal tract in five patients and the upp...