Thanks to their unique optical and electromagnetic properties, noble-metal nanoparticles are proven very useful in many scientific fields, from nanotechnology to biology, including detection of cancer cells. Irradiated gold nanoparticles, as a nano-smolder could be widely used in biomedical contexts such as tumor therapy. Laser destruction of a cancerous tissue depends on thermal and physical p...

Animal models and archived human biobank tissues are useful resources for research in disease development, diagnostics and therapeutics. For the preservation of microscopic anatomical features and to facilitate long-term storage, a majority of tissue samples are denatured by the chemical treatments required for fixation, paraffin embedding and subsequent deparaffinization. These aggressive chem...

W THEN fixed plant tissues are to be sectioned, they are usually dehydrated V V by passing through graded concentrations of alcohol and are then immersed in chloroform to allow infiltration of the cells by paraffin wax. When performed most meticulously, this process may take as long as 9 days; the least time we have been able to use is about 3 days. Moreover, this procedure, especially the trea...

The organ or tissue, e.g., extrinsic eye muscle (Fig. 1), a vermiform appendix (Fig. 2), or a length of blood vessel, is fixed in formalin and impregnated with paraffin wax in the ordinary way. It is removed from the molten wax, laid on a smooth surface, straightened by hand, and allowed to cool. As soon as the wax has become firm a series of transverse cuts are made with a very sharp knife so ...

Background: Clearing is an important step in the preparation of histological sections, that removes alcohol and other dehydrants from tissues prior to infiltration of the embedding material (usually paraffin wax).Different types of clearing agents are chloroform, Xylene, Toluene, Paraffin, Methyl benzoate and methyl salicylate & Citrus fruit oils.The commonly used clearing agent is xylene that ...

numerical analyses have shown that successful flow simulations and the accuracy of solution noticeably depend on the number of nodes used in computational meshing. a suitable meshing should have the capability of adapting with main flow parameters. because the number of total nodes that can be used in numerical simulation is limited, making such grid for complex flows is almost difficult, if it...

Liver transplant biopsies are routinely archived following formalin fixation and paraffin embedding and may provide an additional source of diagnostic information following transcriptomic biomarker analysis. This study was designed to compare gene transcription between resting and stressed biliary cells in culture, these cells after fixation and embedding and archival liver transplant biopsy ti...

The absence of reliable quantitative laboratory tests for measurements of microRNAs and other classes of small noncoding RNAs in archived, formalin- fixed, paraffin-embedded human samples with sufficient specificity and sensitivity has significantly limited the development of clinically relevant noncoding RNA–based diagnostic and therapeutic applications. A report by Renwick et al. in this issu...