In the present work, effectiveness of chlorophyll a fluorescence as quick tool to detect effect Pb–stress on Limbarda crithmoides and Helianthus annuus species, when exposed increasing Pb concentrations (0–500 µmol.L–1) for 45 days, was evaluated. The level in plant leaves, well fresh weight content shoots roots both were also determined. L. did not show any significant variation photochemical ...

PURPOSE This study was designed to investigate the biomechanical properties of nonirradiated (NI) and irradiated (IR) peroneus tendons to determine if they would be suitable allografts, in regards to biomechanical properties, for anterior cruciate ligament reconstruction after a dose of 1.5-2.5 Mrad. METHODS Seven pairs of peroneus longus (PL) and ten pairs of peroneus brevis (PB) tendons wer...

BACKGROUND Lead (Pb) adversely affects neurodevelopment in children. Neural stem cells (NSCs) play an essential role in shaping the developing brain, yet little is known about how Pb perturbs NSC functions and whether such perturbation contributes to impaired neurodevelopment. OBJECTIVES We aimed to identify Pb-induced transcriptomic changes in NSCs and to link these changes to neurodevelopme...

Paspalum fasciculatum Willd. ex FlĂźggĂŠ grows in mining soils which are Cd- and Pb-contaminated where it exhibits tolerance to Pb the ability extract from these soils. To elucidate mechanisms Pb-stress, liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used quantify changes accumulation of proteins leaves. We identified 323 involved primary metabolism response biotic or abioti...

Nicotinamide mononucleotide adenylyltransferase (NMNAT) is a conserved enzyme in the NAD synthetic pathway. It has also been identified as an effective and versatile neuroprotective factor. However, it remains unclear how healthy neurons regulate the dual functions of NMNAT and achieve self-protection under stress. Here we show that Drosophila Nmnat (DmNmnat) is alternatively spliced into two m...

Previous investigations have indicated that de novo protein synthesis is a critical requirement for phenobarbital (PB) induction. We reexamined this issue in PB-responsive primary rat hepatocyte cultures using a broader array of protein synthesis inhibitors and experimental end points. Anisomycin, cycloheximide, emetine, puromycin, and puromycin aminonucleoside, a negative analog, were evaluate...

OBJECTIVES Lead (Pb) is a toxic metal inducing many destructive effects leading to a broad range of physiological, biochemical, and neurological dysfunctions in humans and laboratory animals. MATERIALS AND METHODS Here, we investigated the effect of chronic exposure to Pb (50 mg/l) on oxidative stress, hepatotoxicity, nephrotoxicity, and lipid profile of two different age groups of female rat...

Exported mRNAs are targeted for translation or can undergo degradation by several decay mechanisms. The 5'-->3' degradation machinery localizes to cytoplasmic P bodies (PBs). We followed the dynamic properties of PBs in vivo and investigated the mechanism by which PBs scan the cytoplasm. Using proteins of the decapping machinery, we asked whether PBs actively scan the cytoplasm or whether a dif...

The yeast Saccharomyces cerevisiae is a useful model organism for studying lead (Pb) toxicity. Yeast cells of a laboratory S. cerevisiae strain (WT strain) were incubated with Pb concentrations up to 1,000 μmol/l for 3 h. Cells exposed to Pb lost proliferation capacity without damage to the cell membrane, and they accumulated intracellular superoxide anion (O2 ) and hydrogen peroxide (H2O2). Th...

P-bodies (PB) are ribonucleoprotein (RNP) complexes that aggregate into cytoplasmic foci when cells are exposed to stress. Although the conserved mRNA decay and translational repression machineries are known components of PB, how and why cells assemble RNP complexes into large foci remain unclear. Using mass spectrometry to analyze proteins immunoisolated with the core PB protein Dhh1, we show ...