Cultured corneal epithelial cells release a factor(s) that stimulates trigeminal neurons to form neurites in vitro. To characterize this trophic effect, conditioned media (serum free, supplemented) from cultures for corneal epithelium, stromal fibroblasts, and endothelium were studied further. Only epithelial conditioned medium (PCM) prolonged neuronal survival and induced neurite outgrowth. Th...

Joubert syndrome (JS) is a developmental brain disorder characterized by cerebellar vermis hypoplasia, abnormal eye movement, ataxia and mental retardation. Mutations in CEP290 mutations are responsible for the cerebello-oculo-renal subtype of JS that includes kidney cysts and retinal degeneration, two phenotypes commonly linked to ciliopathies. CEP290 mutations are also associated with Meckel-...

The efficient use of nutrients is important in development and aging. In this study, we asked if the protein repair methyltransferase has a related or additional role in energy metabolism and stress response in the nematode Caenorhabditis elegans. Worms lacking the pcm-1 gene encoding this enzyme exhibit reduced longevity as SDS-isolated dauer larvae and as arrested L1 larvae under starvation s...

For several decades, computer’s memory has been volatile. High speed memories such as DRAM and SRAM have been used as main memory and cache memory respectively. Magnetic disks are used as the persistent secondary storage devices. The present DRAM based main memory has reached its energy limits. Due to the advent of nonvolatile memories like PCM, MRAM, RRAM, FeRAM and Flash, there is growing int...

Centrosomes consist of a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Proteins that contain a Pericentrin/AKAP450 centrosomal targeting (PACT) domain have been implicated in recruiting several proteins to the PCM. We show that the only PACT domain protein in Drosophila (the Drosophila pericentrin-like protein [D-PLP]) is associated with both the centrioles and th...

Phase change memory (PCM) as a newly developed storage medium has many attractive properties such as non-volatility, byte addressability, high density and low energy consumption. Thus, PCM can be used to build non-volatile main memory databases. However, PCM’s long write latency and high write energy bring challenges to PCM-based memory systems. In this paper, we propose an improvement over the...

Nowadays, many applications use spatial data for instance-location information, so storing spatial data is important. We suggest using R -Tree over PCM. Our objective is to design a PCM-sensitive R -Tree that can store spatial data as well as improve the endurance problem. Initially, we examine how R -Tree causes endurance problems in PCM, and we then optimize it for PCM. We propose doubling th...

We describe a new architecture for passive robots and haptic displays, which we call a programmable constraint machine (PCM). An n-dof PCM can, under computer control, exhibit constraints (smooth, impenetrable virtual surfaces of dimensionality < n), or it can allow free n-dof motion. At the heart of the PCM is a nonholonomic element, which is used as a continuously variable transmission (CVT)....

Phase change memory (PCM) is the best candidate device among next generation random access memory technologies. PCM has a potential to replace Flash memory due to non-volatility and in-place programmability, and low power consumption. Even though lifetime of PCM is longer than flash memory, wear leveling is needed because of non-uniformity of storage workload or malicious attack. In this paper,...

The dot immunobinding assay, a rapid, visually read test, was adapted for serodiagnosis and follow-up of paracoccidioidomycosis (PCM). Purified gp43 antigen was tested before and after sodium metaperiodate treatment. To evaluate the assay, it was tested with sera from PCM, histoplasmosis, Jorge Lobo's disease, aspergillosis, candidiasis, and cryptococcosis patients and healthy subjects. Native ...