BACKGROUND Reverse transcription PCR (RT-PCR) and real time RT-PCR (rRT-PCR) have been indispensable methods for influenza surveillance, especially for determination of avian influenza. The movement of testing beyond reference lab introduced the need of quality control, including the implementation of an evaluation system for validating personal training and sample proficiency testing. METHOD...

A real-time reverse transcription multiplex polymerase chain reaction (rRT-MPCR) was developed for detection of mRNA encoded by rfbE and eae genes of enterohemorrhagic Escherichia coli (EHEC) O157:H7. A 129-bp and a 106-bp sequence specific to rfbE and eae, respectively, were targeted for reverse transcription, amplification, and real-time detection. A single-step RT-PCR kit containing a mixtur...

The pandemic COVID-19 needs a rapid microbiological diagnostic from Clinical Microbiology Units. Due to the fact that it is done by using reverse transcription polymerase chain reaction (rRT-PCR) previous RNA extraction and automated equipment reagents for represent an economic increase diagnosis, we describe easy, cost-effective fast alternative extraction-free SARS-CoV-2. Samples were treated...

COVID-19 is a disease caused by SARS-CoV-2, new virus from genus β-coronaviruses. This has been declared pandemic WHO on 11 March 2020 until now. The nucleic acid tests are the most frequently used assays because of their high sensitivity and specificity. One GeneXpert, real-time reverse transcription polymerase chain reaction (rRT-PCR)-based assay platform. use GeneXpert shows great public hea...

Considering the prevailing scenario of COVID-19 pandemic, early detection disease is an important and crucial step in management. Early correct treatment may limit progression to severe levels prevent deaths. In addition, isolation infected patients will lead control transmission rate possibly reduce stress on present healthcare system. Currently, most common reliable testing method available f...

Objective: The low sensitivity of the real-time reverse transcription-polymerase chain reaction (rRT-PCR) test on nasopharyngeal swab for SARS-CoV2 virus could cause infections among health care professionals and be a source viral spread. aim this study was to verify whether lung ultrasonography (LUS) had negative predictive value (NPV) high enough rule out coronavirus disease 2019 (COVID-19) i...

OBJECTIVE Influenza is considered as an emerging disease until today. The present study was undertaken to determine the prevalent genotypes of Influenza A virus in Malaysia. METHODS Influenza A virus was identified from respiratory specimens by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). Phylogenetic analysis of the identified isolates was performed and genotypes were...

We evaluated the performance of four rapid influenza diagnostic test methods (RIDT) compared to real-time reverse-transcription polymerase chain reaction (rRT-PCR), for the detection of the novel swine-origin influenza A (H1N1) virus (S-OIV) in August 2009. A total of 270 respiratory specimens were tested with rRT-PCR, where 74 of these were tested by BinaxNow (Inverness), 80 by QuickVue (Quide...

Due to concerns that high pathogenicity avian influenza would enter into the United States, an interagency strategic plan was developed to conduct surveillance in wild birds in order to address one of the possible pathways of entry. The USDA and state wildlife agencies participated in this effort by collecting samples from 145,055 wild birds from April 2006 through March 2008 in all 50 states. ...

Acute viral gastroenteritis is an intestinal infection that can be caused by several different viruses. Here we describe the evaluation and verification of Seeplex Diarrhea-V ACE (Seeplex DV), a novel commercial multiplex reverse transcription-PCR (RT-PCR) assay that detects 5 diarrheal pathogens, including adenovirus, rotavirus, norovirus genogroup I (GI) and GII, and astrovirus. We describe a...