The performance of different in vitro diagnostic tests for the diagnosis of invasive aspergillosis (IA) was investigated in a transiently neutropenic rat model. Rats were immunosuppressed with cyclophosphamide and then inoculated intravenously with 1.5 x 10(4) CFU Aspergillus fumigatus spores. Animals were then either treated with caspofungin acetate, 1 mg/kg/day for 7 days, or not treated. PCR...

Serum and DNA from blood samples collected from Vietnamese yellow cattle (n=101) and cattle imported from Thailand (n=54) at a Vietnamese slaughter house were screened for Babesia bovis and Babesia bigemina infections by enzyme-linked immunosorbent assay (ELISA) and PCR. The positive rates determined by ELISA (B. bovis and B. bigemina) or PCR (B. bigemina) in the Vietnamese cattle were signific...

BACKGROUND AND OBJECTIVES To assess the cost and effectiveness of adding a system of polymerase chain reaction (PCR) testing to the current enzyme-linked immunosorbent assay (ELISA) screening for hepatitis C virus (HCV) in blood donations. MATERIALS AND METHODS We performed a cost-effectiveness analysis comparing three HCV screening strategies in French blood donors: detection of antibodies a...

The article presents data on the persistence of type 2 circovirus in one farms Republic Belarus. presence pathogen genome pigs different ages was established, and comparative results PCR ELISA were presented.

conclusions based on the findings of this study, s. aureus enterotoxin c (sec) has been detected in synovial fluid of patients with rheumatoid arthritis by pcr and elisa methods. these valuable findings may describe the exact etiology of the ra and as well as change the methods of its diagnosis and treatment. this is the first research, which has shown the staphylococcal entc gene in synovial f...

methods this experimental study was conducted on iranian children communities from may to november 2014. forty stool samples were obtained from laboratories and investigated for heat-labile toxin (lt). specific primers were designed and the dig -labeled pcr products were bounded to streptavidin-coated wells of a microtiter plate and detected by anti-dig-peroxidase conjugate. an internal biotin-...

BACKGROUND The cause of past plague pandemics was controversial but several research teams used PCR techniques and dental pulp as the primary material to reveal that they were caused by Yersinia pestis. However, the degradation of DNA limits the ability to detect ancient infections. METHODS We used for the first time immuno-PCR to detect Yersinia pestis antigens; it can detect protein concent...

BACKGROUND Most current guidelines recommend two serological tests to diagnose chronic Chagas disease. When serological tests are persistently inconclusive, some guidelines recommend molecular tests. The aim of this investigation was to review chronic Chagas disease diagnosis literature and to summarize results of ELISA and PCR performance. METHODS A systematic review was conducted searching ...

Previous studies on Mycoplasma bovis in Poland have used serological methods and culture to isolate the causative organism. PCR and PCR/DGGE molecular tests, antigen ELISA, culture, and antibody ELISA methods were evaluated on 713 animals from 73 Holstein-Friesian herds in Poland with suspected M. bovis infections. The prevalence of M. bovis antibodies in cattle sera was 10.8%. Antigen detectio...

Shiga toxin producing bacteria are potential causes of serious human disease such as hemorrhagic colitis, severe inflammations of ileocolonic regions of gastrointestinal tract, thrombocytopenia, septicemia, malignant disorders in urinary ducts, hemolytic uremic syndrome (HUS). Shiga toxin 1 (stx1), shiga toxin 2 (stx2), or a combination of both are responsible for most clinical symptoms of thes...