Blood culture, the gold standard for diagnosis of neonatal sepsis has low sensitivity and is time consuming. Therefore, objective is development and application of Polymerase chain reaction (PCR)-based DNA sequencing to detect and identify bacterium causing neonatal sepsis in culture negative specimens. Sixty three peripheral blood and 8 cerebrospinal fluid (CSF) referred from tertiary level Ne...

The purpose of this study was to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system. Samples of raw and treated water were collected and concentrated using the membrane filtration technique. Direct Immunofluorescence Test was performed on the samples. DNA extraction using a commercial kit was performed and the DNA extracted was submitted to a ...

To evaluate the sensitivity of high-throughput DNA sequencing for monitoring biowarfare agents in the environment, we analysed soil samples inoculated with different amounts of Bacillus atrophaeus, a surrogate organism for Bacillus anthracis. The soil samples considered were a poorly carbonated soil of the silty sand class, and a highly carbonated soil of the silt class. Control soil samples an...

The most common presentation of animal leptospirosis is the subclinical and silent chronic form, that can lead to important reproductive disorders. diagnosis this form remains a challenge. aim present study gather critically analyse current information about molecular tools applied diagnosis, particularly infection. Regarding clinical specimens, samples from urinary tract were used (69/102, 67·...

Alphavirus species are globally distributed zoonoses primarily transmitted by arthropods. The Venezuelan equine encephalitis virus (VEEV) and Eastern (EEEV) endemic in Costa Rica. This study aims to detect these viruses brain samples from equines displaying nervous signs. For this purpose, four published Universal RT-PCR methods were compared. most sensitive specific method was used test a tota...

Background: DNA vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. Bacteria of the genus Brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. Methods: To evaluate the use of a DNA immunization strategy for protection against brucellosis, a pla...

The DNA of Borrelia burgdorferi spirochetes extracted by ammonium hydroxide was used as the template for nested polymerase chain reaction (PCR) amplification of the species-specific 16S ribosomal DNA (rDNA). The primers were those well known to be specific for signature sequence amplification of the B burgdorferi sensu lato 16S ribosomal RNA gene. The positive 293-base-pair nested PCR amplicon ...

salinity is one of the important problems in the world's agricultural land that each year millions tons of salt from the soil through crop irrigation arrive to arable lands. wheat is one of the first and most important cereal crops in the world that provide staple food for more than a third of the world's population. using modern genetic methods such as sequencing provided quickly and...

The objective of this study is to compare two EGFR testing methodologies (a commercial real-time PCR kit and a specific EGFR mutant immunohistochemistry), with direct sequencing and to investigate the limit of detection (LOD) of both PCR-based methods. We identified EGFR mutations in 21 (16%) of the 136 tumours analyzed by direct sequencing. Interestingly, the Therascreen EGFR Mutation Test kit...