نتایج جستجو برای: pcr sscp
تعداد نتایج: 175795 فیلتر نتایج به سال:
calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study, the polymorphism of the cast gene of makoei sheep was investigated by polymerase chain reaction and single strand conformation polymorphism technique (pcr–sscp). genomic dna was extracted from whole blood samples collected from 100 sheep. a 622 bp cast exon 1 segmen...
ژنهای بخش سوماتوتروپیک شامل عوامل رونویسی هستند که در تکامل و رشد جوجه نقش اساسی دارند. ژن pit1 عامل ویژه رونویسی هیپوفیزی است که نقش عمدهای در تمایز هیپوفیز قدامی و تنظیم ژنهای هورمونهای پرولاکتین، رشد و محرک تیروئیدی دارد. ژن pit1 به عنوان یک ژن کاندیدای کلیدی برای صفات تولیدی طیور مورد توجه قرار گرفته است. این پژوهش به منظور بررسی چندشکلی اگزون ششم ژن pit1 با استفاده از روش چندشکلی فض...
in this study, substitution at codon ser315 of katg gene, a reliable marker for isoniazid (inh) resistance was analyzed and compared by three molecular methods such as dna sequencing, polymerase chain reaction restriction fragment length polymorphism (pcr-rflp) and pcr-single strand conformation polymorphism (pcr-sscp) in 105 phenotypically resistant isolates obtained from various parts of indi...
A fluorescence-based method for polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis, F-SSCP, was developed in which the target sequence is amplified by the PCR using fluorescent primers. The amplified products are then heat-denatured and applied to a water-jacket controlled gel in an automated DNA sequencer. The separated strands are detected as laser-excited f...
We used single-strand conformational polymorphism (SSCP) to determine apolipoprotein E (Apo E) genotypes in 47 individuals. A 295-base-pair (bp) DNA fragment coding for amino acid residues 80-178 of the Apo E protein gave distinct patterns for the three alleles. When we used SSCP to determine the Apo E polymorphism of five individuals whose phenotyping results differed from those of genotyping,...
genotypes of iranian zel sheep for calpastatin(cast) locus were determined by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) and polymerase chain reaction-single strand conformation polymorphism (pcr-sscp) methods and for calpain(capn) locus by pcr-sscp. blood samples were collected from 200 purebred zel sheep of zel breeding station located in golestan province i...
A new molecular biological approach for the identification of bacteria is described. This approach employs PCR of bacterial cell lysates with conserved primers located in the 16S rRNA sequence flanking a variable region, and analysis of the amplified product was based on the principle of single-strand conformation polymorphism (SSCP). The PCR product was denatured and separated on a nondenaturi...
Hepatitis C virus (HCV) populations in vivo consist of heterogeneous mixtures of genetically different but closely related variants defined as a 'quasispecies'. The longitudinal fluctuation of HCV quasispecies populations in chronic hepatitis C has not been elucidated. Serial plasma samples were obtained from four patients with chronic hepatitis C (two patients without any treatment and two pat...
Apolipoprotein E (APOE) gene on chromosome 19q13.2 is encoded by three common alleles designated as epsilon2, epsilon3 and epsilon4. In Alzheimer's disease (AD) the epsilon4 allele is over-represented and is considered to be a major genetic risk factor. Several methods have been developed to determine APOE genotypes. Among them, polymerase chain reaction-single strand conformation polymorphism ...
چکیده زمینه و هدف: بتا تالاسمی یکی از شایع ترین بیماری های ژنتیکی در ایران است و بیش از دو میلیون حامل بتا تالاسمی در ایران وجود دارد. شناسایی جهشهای ژن بتاگلوبین برای برنامههای تشخیصی و مدیریتی معین مانند تشخیص پیش از زایمان بیماری بتاتالاسمی ضروری است. در کشور ما روش PCR-ARMS(PCR-amplification refractory mutation system) بطور گسترده برای شناسایی جهشهای ژن بتا گلوبین استفاده میشود. روش...
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