AIM To assess the association between ABO blood group genotypes and genetic risk factors for thrombosis (FV Leiden, prothrombin G20210A, and methylenetetrahydrofolate reductase C677T mutations) in the Croatian population and to determine whether genetic predisposition to thrombotic risk is higher in non-OO blood group genotypes than in OO blood group genotypes. METHODS The study included 154 ...

Objective This study evaluated the influence of glycemic control on the levels and frequency of subgingival periodontal pathogens in patients with type 2 diabetes mellitus (DM) and generalized chronic periodontitis (ChP). Material and Methods Fifty-six patients with generalized ChP and type 2 DM were assigned according to the levels of glycated hemoglobin (HbA1c) into one of the following gro...

This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S-23S ribosomal in...

The highly polymorphic swine leucocyte antigen (SLA) genes are one of the most important determinants in swine immune responses to infectious diseases, vaccines, and in transplantation success. Study of SLA influence requires accurate and effective typing methods. We developed a simple and rapid method to type alleles at the three classical SLA class I loci (SLA-1, SLA-3 and SLA-2) using the PC...

The entC gene from Escherichia coli which codes for isochorismate synthase (ICS) was introduced into Brassica rapa varieties i.e. var. rapa cv. Raapstelen and ssp. oleifera. Transgenic plants of ssp. oleifera carrying the ICS gene have been developed via Agrobacterium tumefaciens-mediated transformation. This method involved the use of hypocotyl explants co-cultivated with disarmed Agrobacteriu...

BACKGROUND HLA-B*27 is strongly associated with ankylosing spondylitis (AS). It represents a family of alleles that differ among ethnic groups. OBJECTIVE The aim of this study was to determine the distribution of HLA-B*27 alleles in AS patients and healthy controls in Isfahan (Iran). METHODS Sixty AS patients and 430 healthy blood donors were selected. All subjects were HLA-B*27 positive by...

Background: Considering the role of HLA matching in transplant outcome, the quality of HLA-DR typing is clearly an important issue. In recent years, serological methods have been replaced with DNA based typing methods. The main objective of this study was to compare HLA-DR typing data obtained from existing serologic method with data obtained by the new PCR-SSP method. Methods: 55 peripheral bl...

Sea buckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a dioecious windpollinated shrub with nitrogen fixing ability. The berries have bright colors, varying from yellow, orange to red. Domestication of sea buckthorn started in Siberia in the 1930s (Kalinina and Panteleyeva 1987). Local germplasm (ssp. mongolica) from the Altai mountains was used in the onset of the breeding. Breeding project...

The section sensitivity profile (SSP) was well understood in the case of single-row-detector spiral CT. With the introduction of multi-row-detector spiral CT and the transition into cone-beam spiral CT, a revisit to the SSP issue becomes necessary. In this paper, the SSP of multi-row-detector spiral CT is formulated for the half-scan interpolation method at any transverse position. Based on the...

BACKGROUND The aim of this study was to investigate the serology and gene sequence characteristics of the A307 subgroup of the ABO blood group. MATERIAL AND METHODS Monoclonal anti-A and anti-B antibodies were used to detect the ABO antigens of a proband whose positive blood type was not consistent with the negative blood type of the ABO blood group. Standard A-, B-, and O-negative typing cells...