OBJECTIVE To determine whether cerebrospinal fluid (CSF) enterovirus polymerase chain reaction (PCR) testing of febrile neonates is associated with a shorter hospital length of stay (LOS). DESIGN Retrospective cohort study. SETTING Urban tertiary care children's hospital emergency department. PARTICIPANTS Febrile infants 56 days or younger evaluated by means of lumbar puncture. MAIN EXP...

PURPOSE To evaluate the utility of the polymerase chain reaction (PCR) test for diagnosing osteoarticular tuberculosis (TB). METHODS Clinical samples (synovial tissue and synovial fluid) obtained from 23 cases of suspected osteoarticular tuberculosis were subjected to Ziehl Neelsen (ZN) smear examination, radiometric BACTEC culture and PCR test for tuberculosis by amplifying 65 kDa antigen co...

Antimicrobial agents are used for suppressing some bacterial infections during the rearing of poultry. In this study; antibiotic resistance properties Extended Spectrum ßeta-lactamase (ESßL), Amp-C, carbapenemase-producing Escherichia coli (E. coli) strains isolated from chicken breast meat were determined by disc diffusion and E-test methods. Additionally, it is aimed to determine genes classi...

This infographic outlines evidence-based recommendations on COVID-19 reverse transcriptase PCR (RT-PCR) testing in elite sport settings, aiming to protect personal and population health, acknowledging resources expertise that are often available sport. Public health vary by country region, protocol decisions should be made consultation with relevant public authorities. An expert, multidiscip...

AIM To determine the efficiency of HIV DNA PCR (qualitative) and HIV RNA PCR (quantitative) to detect or rule out HIV infection in infants born to HIV infected mothers. SETTING Pediatric and perinatal HIV clinic in a tertiary pediatric hospital. STUDY DESIGN Prospective study METHODS AND MATERIALS 52 infants born to HIV positive mother were tested for HIV infection by HIV DNA PCR or HIV-1...

BACKGROUND Streptococcus pneumoniae is the leading cause of community acquired pneumonia; however, only a small proportion of cases can be detected by conventional methods. The ability of the polymerase chain reaction (PCR) test performed on whole blood samples to identify patients with pneumococcal pneumonia was investigated. METHODS One hundred and fourteen consecutive adult patients with c...

BACKGROUND Efficacy of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and stool PCR was evaluated, before and after treatment, in a country with a high prevalence of H. pylori infection. METHODOLOGY A total of 52 patients with dyspeptic symptoms were included in the study. Antral biopsy was collected during pre- and post-therapy periods for rapid urease test (RUT) and PCR. Simila...

The AMPLICOR cytomegalovirus (CMV) test, a new qualitative assay for the detection of CMV DNA in plasma, was compared to conventional methods and quantitative PCR (Q-PCR) assays by using leukocytes and plasma from 179 blood samples from subjects with AIDS. For the diagnosis of CMV disease, cell-based assays such as a Q-PCR with polymorphonuclear leukocytes (Q-PCR-PMNL) and a pp65 antigenemia as...

The aim of the study was to evaluate effectiveness PCR-RТ method using PCR-RТ-IS test system for etiological diagnosis whooping cough in examination patients with infectious respiratory pathology prolonged and contact persons. 113 children aged from 1 month 17 years, 11 months 29 days, 146 family members were examined. Regardless initial diagnosis, all examined by bacteriological, molecular gen...