Variability in growth rate was observed among S strain isolates of Aspergillus flavus from western Arizona. Isolates were grouped into fast and slow growing biotypes and compared for radial growth, and sclerotia, spore, aflatoxin, and pectinase production. Slow and fast biotypes were distributed equally among three collection areas with 35% of 300 S strain isolates classified as the slow biotyp...

Purification and biochemical characterization of pectinase produced by Aspergillus fumigatus isolated from soil decomposing plant materialsRaphael Emuebie Okonji, Babamotemi Oluwasola Itakorode, Julius Ovie Ovumedia, Odunayo Selimot Adedeji

Macerase, a pectinase-containing enzyme mixture, was used to digest cell walls isolated from cultured pear cells. Following digestion, the reaction mixture was boiled to inactivate enzymes. Addition of soluble aliquots of the mixture to suspension cultures of pear cells led to a rapid and transient production of ethylene by the cells.

In this study, microbial utilization of coffee pulp waste to produce three carbohydrases (cellulase, pectinase, and xylanase) has been investigated. An isolate Listeria sp. ISH-16 which grown in liquid medium containing 0.5% alkali extract can grow well with density reach 107 x 58.5 cell/mL. Further, the culture optimum produced pectinase after 72 hours incubation at 37oC, but for cellulase xyl...

In this study, superparamagnetic iron oxide nanoparticles (MNPs) were synthesized via exposure of fungal cell filtrate from Aspergillus flavus to aqueous ions. The extracellular synthesis MNPs was monitored by UV–Vis spectrophotometry and showed an absorption peak at 310 nm. morphology found be flake-like, as confirmed Field Emission Scanning Electron Microscopy (FESEM), while the average cryst...

A simple procedure for the preparation of plant cell protoplasts is described. The leaf is first treated with pectinase under conditions that do not liberate cells; cellulase is then used to release protoplasts. The yields are comparable to those obtained by methods that entail the prior preparation of free cells. The method is of general application but is particularly useful with tissues that...

Banana besides its nutritive value the biomass can be used as resources for natural fibre. North east India and Assam in particular has a vast potential of extraction and value addition of natural fibre, especially from banana. Banana farming generates huge quantities of biomass most of which goes as waste due to non‐availability of suitable technology for its commercial utilization. Banana fib...

BACK GROUND For enzyme production, the costs of solid state fermentation (SSF) techniques were lower and the production higher than submerged cultures. A large number of fungal species was known to grow well on moist substrates, whereas many bacteria were unable to grow under this condition. Therefore, the aim of this study was to isolate a highly efficient strain of Bacillus sp utilizing wheat...

A total of 48 endophytic bacteria were isolated from surface-sterilized tissues of the medicinal plant Lonicera japonica, which is grown in eastern China; six strains were selected for further study based on their potential ability to promote plant growth in vitro (siderophore and indoleacetic acid production). The bacteria were characterized by phylogenetically analyzing their 16S rRNA gene si...