Yang CR, Raghuram V, Emamian M, Sandoval PC, Knepper MA. Deep proteomic profiling of vasopressin-sensitive collecting duct cells. II. Bioinformatic analysis of vasopressin signaling. Am J Physiol Cell Physiol 309: C799–C812, 2015. First published August 26, 2015; doi:10.1152/ajpcell.00214.2015.—Vasopressin controls osmotic water transport in the renal collecting duct through regulation of aquap...

The redifferentiation, proliferation, and hyaline-specific extracellular matrix (ECM) protein synthesis of chondrocytes cultured in a polycaprolactone (PCL) scaffold were analyzed. Gene expression of the type II collagen and aggrecan was assessed by real-time PCR in cells from PCL scaffolds, monolayer, and pellet cultures. The proliferative activity was assessed using Ki-67 immunodetection, and...

Vasopressin controls osmotic water transport in the renal collecting duct through regulation of aquaporin-2 (AQP2). We carried out bioinformatic analysis of quantitative proteomic data from the accompanying article to investigate the mechanisms involved. The experiments used stable isotope labeling by amino acids in cell culture in cultured mpkCCD cells to quantify each protein species in each ...

In terms of pellet mill operations, the conditioning process has greater influence on pellet quality than does die specification. A great deal of attention must be paid to steam quantity and control, moisture content, retention time and mixing action within the conditioner. In general, most feed manufacturers have not optimized the conditioning process but try to solve pellet quality issues usi...

The description logic SHOIN (D) has attracted considerable interest as the foundation of the W3C standard Web Ontology Language variant, OWL-DL. Pellet is a sound and complete tableau reasoner for SHOIN (D) and incorporates a number of key features such as conjunctive ABox query, axiom pinpointing, rules, E-connection reasoning, and novel optimizations for nominals. In this paper we summarize P...

INTRODUCTION Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into chondrocytes, osteoblasts, myocytes, adipocytes, and a variety of other cell types. Several studies have been directed toward using MSCs from osteoarthritic (OA) patients for cartilage repair not only because these patients are the ones that will require a source of autologous stem cells if biologi...