Filamentous phage display has been extensively used to select proteins with binding properties of specific interest. Although many different display platforms using filamentous phage have been described, no comprehensive comparison of their abilities to display similar proteins has been conducted. This is particularly important for the display of cytoplasmic proteins, which are often poorly dis...

Antibodies are the fastest growing sector of biological therapeutics, and currently more than 400 monoclonal antibodies are in the pipeline (Dübel 2007). The quest to obtain human antibodies for therapy has led many researchers to design diverse display technologies as well as to generate larger and more diverse antibody display libraries (Mondon et al. 2008). Although many different display te...

Autoimmune diseases affect approximately 3% of the population and are a significant cause of morbidity and mortality. Therefore, they are the focus of vivid research aimed at the delineation of pathology, more efficient diagnosis and therapy. Phage display is a simple methodology markedly useful in studying protein-protein interactions which are the driving forces of (patho)physiological proces...

Bovine viral diarrhea-mucosal disease (BVD-MD) is caused by bovine viral diarrhea virus (BVDV), and results in abortion, stillbirth, and fetal malformation in cows. Here, we constructed the phage display vector pCANTAB 5E-VHH and then transformed it into Escherichia coli TG1-competent cells, to construct an initial anti-BVDV nanobody gene library. We obtained a BVDV-E2 antigen epitope bait prot...

The presentation of recombinant peptide libraries linked to their coding sequence can be referred to as 'peptide display'. Phage display is the most widely practiced peptide display technology but more recent alternatives such as CIS display, ribosome display and mRNA display offer advantages over phage for speed, library size and the display of unnatural amino acids. These have provided resear...

This paper presents an analysis of phage-displayed libraries of peptides using Illumina. We describe steps for the preparation of short DNA fragments for deep sequencing and MatLab software for the analysis of the results. Screening of peptide libraries displayed on the surface of bacteriophage (phage display) can be used to discover peptides that bind to any target. The key step in this discov...

Downlo mentous M13 phage can be engineered to display cancer cell–targeting or tumor-homing peptides h phage display. It would be highly desirable if the tumor-targeting phage can also carry anticancer to deliver them to the cancer cells. We studied the evolution of structures of the complexes between c filamentous M13 phage and cationic serum-stable liposomes that encapsulate the monomeric pho...

Abstract TIGIT is an emerging immune checkpoint receptor expressed higher than PD-1 in some tumors especially anti-PD1 resistant tumors. By competing with the co-stimulatory CD226/DNAM-1 for binding to CD155/PVR, transduces inhibitory signals and suppresses response. Therefore, we sought discover inhibitors improving tumor immunotherapy. Through directional cloning, bacterial transformation pha...

Both phage display and ribosome display systems can be used effectively to isolate large numbers of sequence-diverse antibodies from naı̈ve libraries. Once leads have been identified the same display technologies can be used to engineer the characteristics of the drug candidates to yield the desired in vivo efficacy. Ribosome display has a number of advantages over phage display resulting in sig...

Expression of toxic gene products affects bacterial cell growth and phage display, causing a strong selection against plasmid maintenance and integrity. During phage propagation steps, in particular, phagemid instability can dramatically affect diversity of antibody libraries or even lead to the deletion of antibody genes. We constructed a modified phage display vector by introducing a strong t...