Extracts of Phanerochaete chrysosporium cultures grown on birch or on a malt extract-peptone-glucose agar medium were analysed by HPLC. A major component from the two sources appears to be identical by HPLC and UV-visible spectrometry. The product isolated from agar-grown cultures was purified to apparent homogeneity and structure analysis by NMR indicates that the metabolite is the beta-resorc...

A total of 149 species and 209 strains of Korean Aphyllophorales in Seoul National University Fungus Collection (SFC) were analyzed by taxonomic and phylogenetic methods. Among those examined fungal specimens, 9 genera Abundisporus, Antrodiella, Cyphellopsis, Dendrothele, Dichomitus, Laxitextum, Piloderma, Skeletocutis and Tubulicrinis, and 23 species, Abundisporus fuscopurpureus, Antrodiella s...

Analysis of a 2.4-kb cDNA of the cellulose-binding extracellular beta-glucosidase (CBGL) from Phanerochaete chrysosporium suggested that CBGL is organized into two domains, an N-terminal cellulose-binding domain and a C-terminal catalytic domain. Genomic sequence analysis suggested that cbgl is encoded by 30 exons. Southern analysis of DNA from homokaryotic cultures indicated that CBGL is encod...

Phanerochaete flavido-alba is able to remove simple and polymeric phenols from the recalcitrant wastes of the olive oil industry, in a process in which a laccase is involved. This report describes the characterization of a laccase produced by P. flavido-alba and induced by vanillin. Although the amino acid composition of the purified enzyme is typical for laccases, other molecular characteristi...

Heterokaryons made from auxotrophic strains of the lignin-degrading basidiomycete Phanerochaete chrysosporium were induced to fruit. The isolation of wild-type and double-mutant phenotypes from these crosses indicated that genetic recombination had occurred. Cytological studies demonstrated that more than 90% of the basidiospores from the wild-type and auxotrophic strains and from forced hetero...

A preparation of two commercial enzymes was used to liberate protoplasts from 16-h-old mycelium of Phanerochaete chrysosporium. Regeneration frequencies of up to 5% were attained when the protoplasts were plated in a medium containing 10% sorbose and 3% agar. Fusion of protoplasts from different auxotrophic strains in polyethylene glycol-Ca produced heterokaryons. Separation of the heterokaryon...

Benzotriazole and methyl benzotriazole were studied in soil, water, and wood. As reported by various authors, benzotriazole is highly stable in soil or water. It was also found to be a potent inhibitor of plant growth, killing several species at levels of about 0.1 mg/ml. Both benzotriazoles are readily degraded by the Fenton reaction; they are also degraded slowly in the presence of horseradis...

A high degree of lipid oxidative damage was detected in manganese-deficient lignin peroxidase (LIP)-producing cultures of Phanerochaete chrysosporium, indicating intensive exposure to high concentrations of reactive oxygen species (ROS), as is typically found in oxygenated LIPproducing cultures. The absence of manganese ions (Mn2+) in the cultures prevented activation of the manganese-containin...