In referring to various methods for genomic DNA extraction from different tissues, we modified the classical phenol-chloroform procedure and reaction system for use in genomic DNA extraction from pig hair sacs. With the modified the phenol-chloroform method we successfully obtained high quality genomic DNA from pig hair sacs. Genomic DNA can be extracted from sacs of one to six pig hairs with s...

Phenol is one of the organic pollutants in various industrial wastewaters especially petrochemical and oil refining. Biological treatment is one of the considerable choices for removing of phenol present in these wastewaters. Identification of effective microbial species is considered as one of the important priorities for production of the biomass in order to achieve desirable kinetic of biolo...

The effect of phenol on the biological treatment of wastewaters from a resin producing industry was analyzed in a pre-denitrification system. First, the effect of phenol overloads on the removal of organic matter and nitrogen compounds was studied. During the overloads (from 250 to 4000 mg/L), phenol was detected in the effluent of the anoxic reactor but the system recovered fast after stopping...

The mud crab, Panopeus herbstii, was acutely exposed (96-hr) to chlorine-produced oxidants (CPO), phenol, and a CPO-phenolic mixture (1:1) to determine lethal and sublethal effects. The 96-hr (LC50) values were determined for each individual compound and mixture. Additionally, whole-animal respiration rates were measured following acute exposure to sublethal concentrations of each compound or m...

Phenol-formaldehyde/silica hybrid gel with hydrophobic character, high porosity, and thesmall pore size mean was prepared via sol-gel polymerization under solvent saturated vaporatmosphere and was dried by ambient drying method. The silica sols were prepared based onTetraethoxysilane (TEOS) and Methyltrimethoxysilane (MTES) as hydrophilic and hydrophobicprecursors, respectively. Phenol-formalde...

The four transmembrane chemoreceptors of Escherichia coli sense phenol as either an attractant (Tar) or a repellent (Tap, Trg, and Tsr). In this study, we investigated the Tar determinants that mediate its attractant response to phenol and the Tsr determinants that mediate its repellent response to phenol. Tar molecules with lesions in the aspartate-binding pocket of the periplasmic domain, wit...

Benzene, an important industrial solvent, is also present in unleaded gasoline and cigarette smoke. The hematotoxic effects of benzene in humans are well documented and include aplastic anemia, pancytopenia, and acute myelogenous leukemia. However, the risks of leukemia at low exposure concentrations have not been established. A combination of metabolites (hydroquinone and phenol, for example) ...

Due to the advantages of Artificial Neural Network (ANN) for analyzing complex reaction system, the oxidation process of phenol in a pulsed discharge plasma system is simulated using an ANN model. Reaction factors including solution with pH values of 3.6, 5.4 and 9.8, and hydroxyl radicals (·OH) scavengers (Na2CO3 and n-butyl alcohol) are considered, and the changing trends of phenol degradatio...

This study presents the first evidence for the accelerated degradation of phenol by Fenton's reagent in a mini-fluidic VUV/UV photoreaction system (MVPS). A low-pressure mercury lamp used in the MVPS led to a complete degradation of phenol within 4-6 min. The HO˙ and HO2˙ originating from both Fenton's reagent and VUV photolysis of water were identified with suitable radical scavengers. The eff...