Persistence is a prime example of phenotypic heterogeneity, where a microbial population splits into two distinct subpopulations with different growth and survival properties as a result of reversible phenotype switching. Specifically, persister cells grow more slowly than normal cells under unstressed growth conditions, but survive longer under stress conditions such as the treatment with bact...

Despite their evolutionary significance, little is known about the adaptation dynamics of genomically rewired cells in evolution. We have confronted yeast cells carrying a rewired regulatory circuit with a severe and unforeseen challenge. The essential HIS3 gene from the histidine biosynthesis pathway was placed under the exclusive regulation of the galactose utilization system. Glucose contain...

aim : we aimed to develop a multiplex pcr assay for specific detection of epec and ehec pathotypes based on specific marker genes. background : about 2.5 million infant’s morbidity in developing countries occurs by e.coli pathotypes because of diarrhea and intestinal diseases. the traditional phenotypic methods are time consuming and sometimes detection and differentiation of the pathotypes are...

it has been investigated the in vitro antioxidant properties of ethanol extracts of the contained-allantoin plants in this study. contained-allantoined plant samples plantago lanceolata, plantago major, robinia pseudoacacia, platanus orientalis and aesculus hippocastanum were tested at different concentrations. the antioxidant activities of plant samples were analysed by 1,1- diphenyl-2-picrylh...

Temperature-sensitive periods were determined by application of temperature shifts and shocks to 3 temperature-sensitive cell division arrest (cda) mutants of Tetrahymena thermophila. A restrictive temperature, 36 degrees C, was found at which all 3 mutants are fully penetrant, yet other physiological effects are minimal. At this temperature, the temperature-sensitive period of cdaC2 is a uniqu...

The swimming crab Portunus trituberculatus is an economically vital aquaculture species in China and South East Asia. Monosex female culture has been identified as a key priority due to their increased market value, consumer preference enhanced growth performance. However, the lack of rapid reliable sex-linked marker panel hampered application sex control understanding determination species. In...

CCR5 andCXCR4 chemokines receptors are critical coreceptors for the binding ofHIV to specific host cells. Guidelines recommend its assessment in case of virological failure or before prescription ofCCR5 inhibitors. Strategies to assess viral tropismmaybe divided into phenotypic and genotypic assays; registrative trials of CCR5 inhibitors used phenotypic assay, but recently genotypic ones have b...

The aim of this study was to assess the species composition of airborne Staphylococcus spp. in public premises, to determine the methicillin resistance of the isolates and the prevalence of mecA gene, determining resistance to β-lactams. In total 65 Staphylococcus strains were isolated from 54 sites. Four strains exhibited phenotypic methicillin resistance, while the presence of mecA gene was f...

Close monitoring of drug susceptibility among human influenza viruses was necessitated by widespread resistance to M2 inhibitors in influenza H1N1 (pre-pandemic and 2009 pandemic) and H3N2 viruses, and of oseltamivir resistance in pre-pandemic H1N1 viruses. The FDA-approved neuraminidase (NA) inhibitors (NAIs), oseltamivir and zanamivir, as well as investigational NAIs, peramivir and laninamivi...