Experimental myocardial ischemia produced in dogs by proximal left anterior descending coronary artery (LAD) ligation results in a small but measurable decrease in total phospholipid content in the subendocardium of the ischemic region. After 3 hours of fixed occlusion, there is a 10% decrease in total phospholipid content. Silica gel chromatography reveals this decrease to be reflected predomi...

Previously, we have shown that lipid-free apoA-I, when incubated with fibroblasts, will produce lipoproteins of pre-alpha mobility (Asztalos, B. F., et al. 1997. Arterioscler. Thromb. Vasc. Biol. 17: 1630-1636). In order to understand the nature of these pre-alpha particles, we further characterized their lipid content. The pre-alpha particles are high density lipoproteins, having a median dens...

Hyaline membrane disease (HMD) is a disorder primarily affecting premature infants and is a significant cause of morbidity and mortality in this population. The disorder is characterized by widespread atelectasis and an inability of the neonate to expand his or her lungs. Secondary hemor rhage, edema and desquamation of alveolar lining cells then occur which further compromise respira tory func...

The lipids extracted from rice brans were classified by thin-layer chromatography into eight fractions, and their fatty acid (FA) compositions were investigated among five different Japanese cultivars. The lipids of these rice brans comprised mainly triacylglycerols (TAG; 84.9-86.0 wt%), free FA (4.2-4.6 wt%), and phospholipids (PL; 6.5-6.7 wt%), whilst other components were also detected in mi...

Phospholipids of amniotic fluid were extracted, separated by thin-layer chromatography, detected with acidified ammonium sulfate, and charred; the phosphatidyl choline and sphingomyelin fractions were quantitated by transmission densitometry.The densities were recorded as integrator trace units.We used commercially available precoated plates and a solvent system to eliminate the interference of...

Cells of the yeast sterol auxotroph GL7 were grown on either ergosterol or cholesterol to mid-logarithmic phase and total membrane fractions prepared. Activities of phospholipid biosynthetic enzymes in the two cell types were determined. The rates of phosphatidyl-ethanolamine-phosphatidyl-choline-N-methyl transferase and acyl-CoA-alpha-glycerol-3-phosphate transcylase were significantly greater...

1. A number of rumen bacteria isolated because of their ability to deacylate phosphatidyl choline, were found, in addition, to hydrogenate polyunsaturated fatty acids. 2. The most active lipolytic organisms had an unusual pattern of hydrogenation of dietary fatty acids in that alpha-linolenic acid was hydrogenated only as far as trans-11, cis-15-octadecadienoic acid.

OBJECTIVES This study analyzed the antiarrhythmogenic effect of reconstituted high-density lipoprotein (rHDL) against ischemia/reperfusion in vivo. BACKGROUND Recent studies have suggested that a reduction in the plasma HDL level may contribute to cardiac sudden death. Although there are currently only a few therapeutic strategies for increasing HDL, an exciting new therapeutic option, rHDL, ...

Sphingomyelin (SM) is a structural element of cell membranes and lipoproteins, and participates in signal transduction. To determine whether a choline analog (N-amino-N,N-dimethylaminoethanol, N-aminodeanol, NADe) can be substituted for choline in the SM of liver, rats (male, Sprague-Dawley-derived) were fed a diet that was low in choline and methionine, and contained 35.5 mmol of NADe/kg. Afte...

Insoluble fatty surfaces are involved in many important interactions such as in biomembranes with soluble biological macro and micromolecules. In this paper we have studied the adsorption interaction of aqueous solution of DNA, some proteins and lactose on several sparingly soluble fatty substances namely milk fat, stearic acid, palmitic acid, phosphatidyl choline and cholesterol surfaces by me...