This study aimed to evaluate the effects of dietary egg yolk phosphatidylcholine (EPC) and soybean (SPC) on obesity mice fed a high-fat diet (HFD). After 60 days intervention, were evaluated by biochemical indices serum lipidomic analysis. EPC SPC markedly reduced total cholesterol, triacylglycerol (TAG) low-density lipoprotein while increased high-density cholesterol. was more effective in red...

As compared with other methods, our recently reported method for subcellular fractionation of human platelets improves the separation of mitochondria, alpha granules, and lysosomal enzyme activities. The relative purity of these fractions has led us to undertake the present study to compare the subcellular distribution of phospholipids, fatty acids, and platelet factor 3 (clot-promoting) activi...

The effect of castration and administration of testosterone propionate (TP) has been studied in mature monkeys in relation to prostatic and seminal vesicular lipids to castrates on prostatic and seminal vesicular lipids were studied in mature monkeys. Castration decreased prostatic total lipid, total phospholipids and total glyceride glycerols. Among neutral lipid classes only diacyl glycerol w...

Lipid in the intestinal lumen is mainly dietary in origin, but there is also an endogenous component from bile, bacteria, and the mucosa (through exudation and cell loss). Perfusion experiments in fasting rats demonstrate that exfoliated cells carry with them into the small intestinal lumen an average of 1.12 mg lipid/30 minutes; lipid classes consisting of phosphatidyl choline (lecithin), trig...

PURPOSE To understand the difference between macular and peripheral regions, tissue samples of human retina, retinal pigment epithelium, and Bruch's membrane/choroid were dissected and analyzed for lipid composition. METHOD To facilitate dissections and enhance the recovery of tissues, eyecups were prefixed for 1 hour in 10% formalin (pH 7). Lipids were extracted from 4-mm trephined punches o...

Effects of ethanol treatment on Leydig cell NADPH-generating enzymes and lipid profiles were studied. Ethanol treatment (3.0 g/kg. b.wt.) twice daily as a 25% (v/v) aqueous solution given to adult Wistar rats reduced the body weight, testis weight and relative weights of the seminal vesicles and ventral prostate. Serum LH and testosterone were also decreased. Similarly, the NADPH-generating enz...

The effect of acetylcholine on the incorporation of P(32) into the individual phosphatides in slices of various structures of the nervous system has been studied. There was a marked stimulation of P(32) incorporation into phosphoinositide and phosphatidic acid, but not into phosphatidyl choline and phosphatidyl ethanolamine, in the cat stellate and celiac ganglia in vitro. Acetylcholine stimula...

This paper discusses a calcium-dependent inactivation of alamethicin-induced conductance in asymmetric lipid bilayers. The bilayers used were formed with one leaflet of phosphatidyl ethanolamine (PE) and one of phosphatidyl serine (PS). Calcium, initially confined to the neutral lipid (PE) side, can pass through the open alamethicin channel to the negative lipid (PS) side, where it can bind to ...

A 10% rat liver homogenate in 0.25 M sucrose-10-3 M ethylenediaminetetraacetate was fractionated into mitochondria, microsomes, and a supematant fraction. Liver mitochondria derived from rats injected with leucine-3H and with phosphate-32P or with glycerolJ4C were incubated with nonlabeled microsomes in the presence of supernatant or of sucrose-EDTA. Labeled microsomes were similarly exposed to...

The composition of the phospholipids and of the total phospholipid fatty acids was determined in the plasma of 10 normal subjects. In addition the fatty acid composition of the plasma phosphatidyl ethanolamine, phosphatidyl serine, lecithin, sphingomyelin, and lysolecithin of 6 of the subjects was measured. A wide array of fatty acids was found in the plasma total phospholipid similar to that f...