The effect of ethanol on receptor-mediated phospholipase C-linked signal transduction processes was investigated in isolated rat hepatocytes. Pretreatment of the cells with ethanol (6-300 mM) markedly inhibited a subsequent stimulation of phospholipase C by vasopressin, angiotensin II, or epidermal growth factor. By contrast, the effects of the alpha 1-adrenergic agonist phenylephrine and of gl...

Using a commercially available test for LDL cholesterol (Boehringer Mannheim), a method was developed for determination of LDL apolipoprotein B without using ultracentrifugation. The infranatant obtained by precipitation of serum with polyvinylsulphate was redissolved in a saline citrate solution and incubated with phospholipase A2, phospholipase C, phospholipase D or triglyceride lipase, respe...

Abstract In this research, the impacts of various enzymes (phospholipase-A 2 (0.3% v/v), lipase (0.03% w/v), and protease (0.5% w/v)) on physico-chemical functional characteristics treated egg white protein (EWP) were determined. The pH, turbidity, colour (L*, a*, b*, ∆E*), gas concentrations in package, relative foaming capacity (RFC), stability analysed during storage at 4 °C. (1,000 ± 60.82)...

Cultures of embryonic chick muscle cells grown in medium containing phospholipase C from Clostridium perfringens incorporated [‘Hlcholine into lipid at a rate 3to &fold higher than control cultures. To determine the mechanism by which stimulation of phosphatidylcholine synthesis occurred in phospholipase C-treated cells, activities of enzymes and levels of intermediates in the biosynthetic path...

Phospholipase C activity and diglyceride lipase activity were studied in the renal cortex and medulla of 10- and 40-week-old stroke-prone spontaneously hypertensive rats (SHRSP) and age-matched normotensive Wistar-Kyoto rats (WKY). Enhanced phospholipase C activity was found in the cortical and medullary cytosol of kidney from SHRSP, and microsomal diglyceride lipase in SHRSP also increased. In...

Since the classical, pioneering studies of Beaufay and De Duve [ 11, it has been known that glucose-6phosphohydrolase (G-6-Pase) is a phospholipid-dependent enzyme. However, the specificity of this dependence is still unknown. Duttera et al. [2] found that Cl. welchii phospholipase C, which hydrolyses microsomal phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin, causes 8...

Davie, Joseph M. (Indiana University, Bloomington), and Thomas D. Brock. Action of streptolysin S, the group D hemolysin, and phospholipase C on whole cells and spheroplasts. J. Bacteriol. 91:595-600. 1966.-The effect of streptolysin S, the group D hemolysin, and phospholipase C (the alpha toxin of Clostridium perfringens) on whole cells and spheroplasts or protoplasts of three strains of strep...