The cry-b sequence, encoding a basic elicitin (cryptogein B) from Phytophthora cryptogea, was co-transformed into Phytophthora infestans. The copy number of the cry-b sequence varied in co-transformants. Nevertheless, in all cases the alien elicitin gene was transcribed, translated and the protein secreted in vitro from such transformants. Moreover, the secreted cryptogein B from P. infestans c...

Este trabalho descreve a construção e teste de um sistema simples de extração pressurizada com solvente (PSE). Uma mistura de acetona:água (80:20), a 80 oC e 103,5 bar, foi utilizada para extrair dois herbicidas (Diuron e Bromacil) de uma amostra de solo contaminado. Os herbicidas foram identificados e quantificados por cromatografia líquida de alta eficiência acoplada a detector por arranjo de...

Small RNAs, including microRNAs (miRNAs) and phased small interfering RNAs (phasiRNAs; from PHAS loci), play key roles in plant development. Cultivated soybean, Glycine max, contributes a great deal to food production, but, compared to its wild kin, Glycine soja, it may lose some genetic information during domestication. In this work, we analyzed the sRNA profiles of different tissues in both c...

Tomato agar and soybean agar were found to be comparable to or in some cases better than the popular V8 vegetable juice agar in supporting linear growth of Phytophthora cactorum, Phytophthora capsici, Phytophthora parasitica, Pythium aphanidermatum, and Pythium splendens; sporangium production of P. capsici, P. palmivora, and Pythium splendens; and oospore formation of P. cactorum, P. parasitic...

trichoderma species are among important antagonists of plant pathogenic fungi. the main purpose of this study was to evaluate the biocontrol potential of native trichoderma spp. isolates from sugar beet fields against phytophthora drechsleri and p. cryptogea, the causal agents of phytophthora root rot of sugar beet. during 2007–08, 85 isolates of trichoderma spp. from sugar beet fields in eight...

Water molds of the genus Phytophthora include many plant pathogens responsible for epidemics such as potato blight and sudden oak death, causing global economic damages. Sexual reproduction is of biological importance in Phytophthora and has been believed to be stimulated by unknown endogenous factors named a hormones. We describe here the chemical characterization of a Phytophthora mating horm...

Oomycetes, or water moulds, are fungal-like organisms phylogenetically related to algae. They cause devastating diseases in both plants and animals. Here, we describe seven oomycete species that are emerging or re-emerging threats to agriculture, horticulture, aquaculture and natural ecosystems. They include the plant pathogens Phytophthora infestans, Phytophthora palmivora, Phytophthora ramoru...

To date the majority of work on the systemic fungicide phosphite (phosphonate) to control Phytophthora diseases has been conducted on horticultural crops. There is a paucity of work on the control of Phytophthora root and collar rots in natural plant communities. This paper gives an overview of studies conducted in Western Australia which examine the potential of using phosphite in plant commun...

In order to limit the incidence and severity of diseases caused by Phytophthora, effective management strategies are needed. Management of phytophthora diseases is based on a number of principles such as avoiding infection through basic hygiene, limiting susceptibility through drainage and irrigation, improving soil health, use of disease-resistant germplasm, and biological and chemical control...

Phytophthora is a microbial genus that includes species causing many diseases (Erwin and Ribeiro, 1996). These species infect and kill nearly all species of deciduous fruit trees. Phytophthora crown and root rots, which are caused mainly by P. cactorum, P. megasperma, P. citrophthora, P. cinnamomi, P. cambivora, P. syringae and P. cryptogea (Sarejanni, 1935; Kouyeas, 1971; Kouyeas, 1977; Flores...