Forty-two, one-day-old specific pathogen-free (SPF) chickens were randomly divided into two groups. On 21st day of study, experimental group was infected intraocularly with 1×106 EID50 of H9N2 and intratracheally with 1× 1010 CFU of Ornithobacterium rhinotracheale (ORT). Control group was inoculated with sterile PBS intraocularly. The samples from various tissues were collected on days 2, 4, 6,...

Prodrugs of the short-lived chloroethylating agent 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)hydrazine (90CE) and its methylating analogue 1,2-bis(methylsulfonyl)-1-(methyl)hydrazine (KS90) are potentially useful anticancer agents. This class of agents frequently yields higher ratios of therapeutically active oxophilic electrophiles responsible for DNA O(6)-guanine alkylations to other electroph...

widespread occurrence of h9n2 low pathogenic avian influenza (lpai) viruses in many asian countriesduring the past decade has resulted in the need for evaluation of the pathogenesis of h9n2 virus infection. in this study, tissue tropism and dissemination of a/chicken/iran/772/1998(h9n2) virus throughout the body of broiler chickens were investigated. the clinical signs, gross lesions and antibo...

Life cycle of Eimeria krijgsmanni-like coccidium isolated from the feces of naturally infected mice purchased from commercial sources was examined. The parasite was purified by single oocyst isolation and maintained by passage in the mice before experiments. The sporulated oocysts were ovoid or ellipsoid, measuring 19.3 x 14.8 microm on average. One or two small polar granules were present. Mic...

BACKGROUND HIV protease inhibitor (PI)-induced inflammatory response plays an important role in HIV PI-associated dyslipidemia and cardiovascular complications. This study examined the effect of berberine, a traditional herb medicine, on HIV PI-induced inflammatory response and further investigated the underlying cellular/molecular mechanisms in macrophages. METHODOLOGY AND PRINCIPAL FINDINGS...

In order to analyze the effects of inorganic phosphate (Pi) on the energy metabolism of suspen­ sion-cultured cells o f Catharanthus roseus, short-term changes in levels of glycolytic m etabolites were monitored after the addition o f 1.25 mM Pi to cultures of cells previously cultured in a Pi-free M urashige-Skoog medium for 24 h. The levels of all phosphorylated intermediates o f glycolysis e...

Rolett, Ellis L., Ali Azzawi, Ke Jian Liu, Martin N. Yongbi, Harold M. Swartz, and Jeff F. Dunn. Critical oxygen tension in rat brain: a combined P-NMR and EPR oximetry study. Am J Physiol Regulatory Integrative Comp Physiol 279: R9–R16, 2000.—The relationship between cerebral interstitial oxygen tension (PtO2) and cellular energetics was investigated in mechanically ventilated, anesthetized ra...

Toxoplasma gondii KI-1, a recent new isolate from Korea, shows similar pathogenicity and infectivity to mice compared to the virulent RH strain. To understand characteristics of host immunity, including immune enhancement or suppression, we investigated proliferative responses and phenotypes of spleen cells. In addition, kinetics of IFN-γ, a Th1 cytokine, was examined in BALB/c mice up to day 6...

The planar molecules of 6-hydroxy-1,3-benzoxathiol-2-one, C(7)H(4)O(3)S, are linked by extensive O-H.O and C-H.O hydrogen bonding and are further stablilized by face-to-face pi-pi interactions.