The internal ribosomal entry site (IRES)from encephalomyocarditis virus (EMCV) is a popular RNA element used widely in experimental and pharmaceutical applications to express proteins in eukaryotic cells or cell-free extracts. Inclusion of the wild-type element in monocistronic or bicistronic messenger RNAs (mRNAs) confers a high level of cap-independent translation activity to appropriately co...

AIM To examine the physiological role of the androgen receptor (AR) in the PC-3 cell line by transfecting full-length functional AR cDNA driven by its natural human AR promoter. METHODS We generated an AR-expressing PC-3(AR)9 stable clone that expresses AR under the control of the natural human AR promoter and compared its proliferation to that of the PC-3(AR)2 (stable clone that expresses AR...

Mycobacterium avium subsp. paratuberculosis (Map) is the causative agent of johne's disease whose immunopathology mainly depends on cell mediated immuneresponse. Genome sequencing revealed various PPE (Proline-Proline-Glutamic acid) protein family of Map which are immunologically importance candidate genes In present study we have developed a bicistrionic construct pIR PPE/IFN containing a 34.9...

Este artigo pretende analisar, sob uma perspectiva interdisciplinar que congrega os estudos da comunicação e do discurso, textos humorísticos obra Patacoadas, de Cornélio Pires. A pretensão é analisar como objetos complexos entrelaçam questões diversas ordens (composicionais, autorais etc.), tendo objetivo principal investigar a representação caipira com base na noção ethos discursivo (MAINGUEN...

The Hermite-Korkine-Zolotarev reduction plays a central role in strong lattice reduction algorithms. By building upon a technique introduced by Ajtai, we show the existence of Hermite-Korkine-Zolotarev reduced bases that are arguably least reduced. We prove that for such bases, Kannan’s algorithm solving the shortest lattice vector problem requires d d 2e (1+o(1)) bit operations in dimension d....