A simple method for atomic force microscopy (AFM) of nematode cuticle was developed to visualize the external topography of Helicotylenchus lobus, Meloidogyne javanica, M. incognita, and Xiphinema diversicaudatum. Endospores of two isolates of the nematode parasite, Pasteuria penetrans, adhering to M. incognita and X. diversicaudatum were also visualized and measured by this technique. Scanning...

Plants and animals produce reactive oxygen species (ROS) in response to infection. In plants, ROS not only activate defense responses and promote cell death to limit the spread of pathogens but also restrict the amount of cell death in response to pathogen recognition. Plants also use hormones, such as salicylic acid, to mediate immune responses to infection. However, there are long-lasting bio...

Spatial patterns of Meloidogyne incognita, Tylenchorhynchus claytoni, Helicotylenchus dihystera, and Criconemella ornata were analyzed using Hill's two-term local quadrat variance method (TTLQV), spectral analysis, and spatial correlation. Data were collected according to a systematic grid sampling plan from seven tobacco fields in North Carolina. Different estimates of nematode cluster size we...

In a survey of plant-parasitic nematodes associated with agricultural crops in nine Maine counties, 744 soil samples from 26 potential host plants were analyzed between November 1987 and January 1989. The most commonly encountered nematode genus was Pratylenchus, occurring in 85% of the samples from most crops, except blueberries and onions. Pratylenchus penetrans and P. crenatus were found com...

Several species of plant parasitic nematodes have been encountered in tea soils in different tea-growing areas of the world. Roots and soil were sampled for the presence of plant-parasitic nematodes from tea plantations in Kusuku, the only major tea growing location in Nigeria. Six genera and species of plant-parasitic nematodes were recovered from the rhizosphere soil. Meloidogyne spp., Pratyl...

Current knowledge of steroid nutrition, metabolism, and function in free-living, plant-parasitic and animal-parasitic nematodes is reviewed, with emphasis upon recent investigation of Caenorhabditis elegans. A number of 4-desmethylsterols with a trans-A/B ring configuration can satisfy the steroid nutritional requirement in C. elegans, but sterols with a cis-A/B ring configuration or trans-A/B ...

Plant-parasitic nematodes are destructive plant pathogens that cause significant yield losses. They induce highly specialized feeding sites (NFS) in infected plant roots from which they withdraw nutrients. In order to establish these NFS, it is thought that the nematodes manipulate the molecular and physiological pathways of their hosts. Evidence is accumulating that the plant signalling molecu...

Plant parasitic nematodes are microscopic roundworms which live in the soil and attack the roots of plants. Crop production problems induced by nematodes therefore generally occur as a result of root dysfunction, reducing rooting volume and foraging and utilization efficiency of water and nutrients. Many different genera and species of nematodes can be important to crop production in Florida. I...

Communities of plant-parasitic nematodes collected from five different vegetation types (canopy woodland, savannah, gallery forest, cultivated perennial, and annual plants) and soils (yellowish red latosols, dark red latosols, arenosols, acrisols, and gleysols) were studied. Ninety percent of the soil samples collected from savannah contained at least four genera of plant-parasitic nematodes. T...