Graham, T. E., V. Sgro, D. Friars, and M. J. Gibala. Glutamate ingestion: the plasma and muscle free amino acid pools of resting humans. Am. J. Physiol. Endocrinol. Metab. 278: E83–E89, 2000.—Monosodium glutamate (MSG) ingestion is known to increase plasma glutamate concentration, and MSG infusion stimulates insulin secretion. We investigated the impact of MSG ingestion on both the plasma and i...

The special features of nitrogen metabolism in ruminants, which stem from the extensive breakdown and synthesis of dietary protein in the rumen (see Hungate, 1966), imply the absence of a specific dietary requirement for essential amino acids. Nevertheless, there is considerable theoretical and practical interest in defining the amino acid requirements of ruminant tissues in animals of known nu...

1. Anterior tibial muscle protein synthesis in seven healthy postabsorptive men was determined from increases in muscle protein bound leucine enrichment during a primed continuous infusion of L-[1-13C]leucine. Biopsies were taken 30 min after the beginning of leucine infusion (when plasma 13C enrichment was steady), 240 min later during continued fasting and again after 240 min of infusion of a...

that amino acids from tumor protein breakdown may be used as precursors for the synthesis of new protein in tumor cells. Tumor cells have the ability to concentrate amino acids from the plasma amino acid pool more efficiently than do normal cells, thus enabling them to compete successfully with the tissues of their host for available amino acids (17). During times of reduced dietary intake, res...

Portal-systemic shunting of blood is associated with hyperammonemia, an increased glutamine concentration in brain, an altered plasma neutral amino acid pattern, and high levels of several of the large neutral amino acids in brain. Since some of these amino acids are precursors for neurotransmitters and for other potentially neuroactive substances, high CNS levels of these amino acids may contr...

We have measured rates of uptake of arginine, glutamine, glutamate, serine, phenylalanine and glycine in Xenopus laevis oocytes cultured for periods of up to 24h in saline in the presence or absence of a mixture of 20 amino acids at concentrations approximating those in Xenopus plasma. Amino acid supplementation increased the total intracellular amino acid concentration from 8.2 to 18.4 nmol pe...

BACKGROUND Determination of the plasma concentrations of arginine and other amino acids is important for understanding pathophysiology, immunopathology and nutritional supplementation in human disease. Delays in processing of blood samples cause a change in amino acid concentrations, but this has not been precisely quantified. We aimed to describe the concentration time profile of twenty-two am...

study was undertaken to establish the optimum dietary indispensable amino acid (IAA) profile for yellow fin seabream broodstock based on the amino acid (AA) profile of broodstock, eggs and larvae of Acanthopagrus latus. Three isonitrogenous (40%) and isolipid (20%), named diets 1, 2 and 3 were formulated with different ratios of total essential amino acids per total non-essential amino acid...

BACKGROUND Few studies exist on the use of metabolic profiling of amino acids to examine underlying physiologic and disease states. OBJECTIVE We aimed to introduce a new method for studying relations among amino acids and to generate a diagnostic index, or amino index, based on amino acid concentrations. DESIGN For network analysis, 35 Fischer-344 rats were randomly divided into 7 groups an...

We measured amino acid concentrations in plasma and skeletal muscle of three groups of patients with acute hemorrhagic pancreatitis: (a) patients without secondary organ lesions, (b) patients also suffering from kidney damage, and (c) patients in whom the pancreatitis was accompanied by sepsis and multiple organ failure. In all three groups, especially the third group, the amino acid concentrat...