background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verifica...

A fermentation process for production of super coiled plasmid DNA in E. coli for use as a DNA vaccine was developed. In the process a feed-back control nutrient feeding strategy based on pH and DO was used to regulate the cell growth rate by controlling the interactivity of the nutrient feed rate, pH and DO. The process increased the total yield of plasmid DNA by approximately 4 fold as compare...

Gene therapy aims to silence an oncogene through RNA interference, or replace abnormal tumour suppressor via gene augmentation. In this study, we intended interference for PRKCA and augmentation PTEN with a view reduce growth in mouse model of breast cancer. Inorganic carbonate apatite nanoparticles (CA NPs) were utilized deliver the synthetic siRNA purified gene-carrying plasmid DNA both vitro...

Background: Tuberculosis (TB) remains as a major cause of death around the world. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning of tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryotic cells. ...

Introduction: Neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. Also, neurotropic factors are growth factors like Neurotrophins that help neurons survive. Moreover, Neurotrophins differentiate between progenitor cells so that neurons are formed. Despite the fact that the majority of mammalian brain neurons are produced p...

Antibiotic resistance of Salmonella species is well reported. Ciprofloxacin the frontline antibiotic for salmonellosis. The repeated exposure to ciprofloxacin leads resistant strains. After 20 cycles exposure, bacterial clones were evaluated. colony size mutants was small and had an extended lag phase compared parent strain. whole genome sequencing showed 40,513 mutations across genome. Small p...

several heavy metal resistant bacterial strains were isolated from sediment and water samples collected from the persian gulf and enclosed industrial areas. all the isolated bacteria were identified by 16s rrna gene sequencing. isolated bacteria were tested for the presence of plasmids using the modified alkaline lysate method. the method was effective for identification and characterization of...