We explore the use of magnetic relaxation sensors as an in vitro assay platform. Functionalized magnetic nanoparticles that exhibit transverse relaxation time (T(2)) changes with the introduction of an analyte have previously been used to detect proteins, antibodies, receptor ligands, peptides, nucleic acids, oligonucleotides, and other small molecules. The standard procedure for sensitizing pa...

Vaksin DNA telah menjadi bidang penelitian yang menarik dalam upaya melawan penyakit infeksius pada hewan. adalah formulasi vaksin menggunakan fragmen sebagai antigen. mengandung informasi genetik diambil dari agen penyakit, seperti virus atau bakteri, ingin ditargetkan. Penelitian ini bertujuan untuk mengembangkan hewan dan manusia. Metode klon plasmid dengan gen nukleoprotein Covid-19 ditrans...

Clostridium perfringens type A, is both a ubiquitous environmental bacterium and a major cause of human gastrointestinal disease, which usually involves strains producing C. perfringens enterotoxin (CPE). The gene (cpe) encoding this toxin can be carried on the chromosome or a large plasmid. Interestingly, strains carrying cpe on the chromosome and strains carrying cpe on a plasmid often exhibi...

As a prerequisite for a human clinical trial using interleukin (IL)-12 gene therapy, the biodistribution and safety of IL-12, administered as an intradermal naked DNA injection, was evaluated in mice. The pNGVL3-mIL12 plasmid used in this study is a nonviral vector designed to induce a high level of IL-12 protein expression during a transient transfection of the host cell. The biodistribution w...

Two novel complexes [CuL2(H2O)2]n (1), [(ZnL2)H2O]n (2) (HL ?= ?5-(Pyrazol-1-yl)nicotinic acid) were synthesized and their vitro biological activity was systematically studied. The ability of the to bind FS-DNA measured by fluorescence spectroscopy, Stern-Volmer quenching values complex 1–2 are 0.03904 0.09522. cleavage behavior agarose gel electrophoresis showed cut pBR322 plasmid DNA. Both fl...

OBJECTIVES To determine the complete nucleotide sequence of the novel Johannesburg β-lactamase-encoding gonococcal plasmid (pEM1) and to determine the strain relatedness of Johannesburg plasmid-containing penicillinase-producing Neisseria gonorrhoeae (PPNG) by molecular typing. METHODS Eleven PPNG isolates containing the Johannesburg β-lactamase-encoding plasmid were previously identified amo...

To characterize protein-DNA interactions involved in the initiation of conjugative transfer replication, we isolated and sequenced the transfer origins (oriT) of the promiscuous IncP plasmids RP4 and R751. The central initiating event at the transfer origin of a conjugative plasmid is the cleavage at a unique site (nic) of the strand to be transferred to a recipient cell. This process can be tr...

Soluble epoxide hydrolase (sEH) is a multifunctional protein encoded by the EPHX2 gene. The biological functions and enzyme kinetics of sEH have been extensively investigated, however, little is known about its transcriptional regulation and mechanisms of tissue specific expression. Here, a luciferase gene based reporter assay was used to identify the minimal promoter and cis regulatory element...

The rapid spread of antibiotic resistance - currently one of the greatest threats to human health according to WHO - is to a large extent enabled by plasmid-mediated horizontal transfer of resistance genes. Rapid identification and characterization of plasmids is thus important both for individual clinical outcomes and for epidemiological monitoring of antibiotic resistance. Toward this aim, we...