Interferon production was induced in a strain of human diploid foreskin cells with poly(I).poly(C). Cycloheximide was included in the culture medium at the time of addition of the inducer. Actinomycin D was added to the cultures 4 or 5 hr later, before the inhibition of protein synthesis was reversed at 6 hr. Thus, subsequent interferon synthesis had to be directed by messenger RNA synthesized ...

Oblivious low-distortion subspace embeddings are a crucial building block for numerical linear algebra problems. We show for any real p, 1 ≤ p < ∞, given a matrix M ∈ R with n ≫ d, with constant probability we can choose a matrix Π with max(1, n)poly(d) rows and n columns so that simultaneously for all x ∈ R, ‖Mx‖p ≤ ‖ΠMx‖∞ ≤ poly(d)‖Mx‖p. Importantly, ΠM can be computed in the optimal O(nnz(M)...

The poly(dA) dependent T4 polynucleotide ligase catalyzed polymerization of oligodeoxythymidylates is dependent upon duplex stability. The antibiotics ethidium bromide, netropsin and Hoechst 33258 stabilize the duplex poly(dA) . P(dT)n (n = 6-10) to thermal denaturation. Ethidium bromide to DNA ratio of 1.25 and netropsin or Hoechst 33258 to DNA ratio of 0.1 the Tm of d(pT) 10 . poly (dA) was i...

Nucleosomal core particles containing the right- and left-handed conformations of DNA were examined for their ability to support the B----Z or Z----B transition. Nucleosomes were assembled onto the B-and Z-conformations of poly[d(Gm5C)] and the B-conformation of poly[d(GC)] as previously described. Absorbance and circular dichroic spectroscopy indicated that the DNA on all three core particle p...

To identify genes involved in poly(A) metabolism, we screened the yeast gene deletion collection for growth defects in the presence of cordycepin (3'-deoxyadenosine), a precursor to the RNA chain terminating ATP analog cordycepin triphosphate. Deltapho80 and Deltapho85 strains, which have a constitutively active phosphate-response pathway, were identified as cordycepin hypersensitive. We show t...

Endocytosis of 125I-mannose-bovine serum albumin (BSA) and exocytosis of 125I-mannose-poly-D-lysine by rabbit alveolar macrophages were examined as a function of temperature. A plot for total ligand uptake (cell-associated ligand plus degraded ligand) versus time shows a single inflection point at 20 degrees C. Ligand degradation does not occur below 20 degrees C. Internalization of surface-bou...

A long line of research on fixed parameter tractability integer programming culminated with showing that programs $n$ variables and a constraint matrix dual tree-depth $d$ largest entry $\Delta$ are solvable in time $g(d,\Delta){poly}(n)$ for some function $g$. However, the is not preserved by row operations, i.e., given program can be equivalent to another smaller tree-depth, thus does reflect...

We study the problem of clustering sensitive data while preserving the privacy of individuals represented in the dataset, which has broad applications in practical machine learning and data analysis tasks. Although the problem has been widely studied in the context of lowdimensional, discrete spaces, much remains unknown concerning private clustering in highdimensional Euclidean spaces R. In th...

An N-acetyl-beta-D-glucosaminyltransferase activity involved in the initiation of poly-N-acetyllactosamine chain biosynthesis can be solubilized from Ehrlich ascites tumor cell microsomal membranes. The ability of this enzyme to act on linear and branched acceptor substrates has been studied. The results indicate that complex-type tri- and tetra-antennary oligosaccharides exhibiting the branchi...