On the basis of serotyping 62 strains of Bordetella pertussis, Guiso and colleagues (N. Guiso, C. H. Wirsing von König, C. Becker, and H. Hallander, Letter, J. Clin. Microbiol. 39:1684– 1685, 2001) concluded that monoclonal and polyclonal reagents showed good concordance but that the source of the monoclonal antibodies needed to be specified. We have conducted a similar study on 209 strains of ...

Antibodies play an important role in the natural immune response to invading pathogens. The strong and specific binding to their antigens also make them indispensable tools for research, diagnostics and therapy. This thesis describes the development of methods for characterization of antibody specificity and the use of these methods to investigate the polyclonal antibody response after immuniza...

Supplemental Experimental Procedures Reagents and antibodies Reagents were purchased as follows: DRB, wortmannin, NAC, doxycycline, BHA, polybrene and H2O2 were from Sigma, and PD98159 from Calbiochem. Polyclonal anti-phospho-AKT, antiAKT, anti-phospho-S6K, anti-phospho-ATF2, anti-ATF2, anti-phospho-p38, anti-p38, antiphospho-IKKα/β and anti-JNK antibodies were from Cell Signalling. Anti-actin,...

An intestinal protozoan parasite, Cryptosporidium parvum, is a major cause of waterborne gastrointestinal disease worldwide. Detection of Cryptosporidium oocysts in potable water is a high priority for the water treatment industry to reduce potential outbreaks among the consumer populace. Anti-Cryptosporidium oocyst polyclonal and monoclonal antibodies were tested as capture and detection reage...

An emphasis on cellular immunity against Rickettsia has led to neglect of analysis of the role of antibody. The availability of an excellent mouse model of spotted fever rickettsiosis enabled investigation of a potential role of antibody in immunity to Rickettsia conorii. C3H severe combined immunodeficiency (SCID) mice were passively transfused with monoclonal antibodies against rickettsial ou...