Homogeneous cytosolic pyruvate kinase from endosperm of germinating castor oil (Ricinus communis L. cv Hale) seeds was potently activated by polyethylene glycol. The addition of 5% (w/v) polyethylene glycol to the pyruvate kinase reaction mixture caused a 2.6-fold increase in maximal velocity and 12.5- and 2-fold reductions in Km values for phosphoenolpyruvate and ADP, respectively. Glycerol, e...

background: nowadays colonoscopy has a very important role in screening colorectal cancers. in addition, adequate bowel preparation is critical to colonoscopy, as a poor preparation may obscure pathology or prevent full colonoscopy. as it is estimated that unsuccessful colonoscopy leads to second procedures in up to 20% of patients, we sought to compare the effects of different doses of polyeth...

background: fusion of dendritic cells (dcs) with melanoma cells could reinforce the antigenicity of tumors as a strategy for the treatment of malignant melanoma. however, the insufficient quantity of dcs and the low fusion efficiency limits the development of such approach. objective: to define the dosage of the stimulating factors as well as the induction condition for the optimal dcs preparat...

Polyethylene glycol was easily grafted to silica gel and used as a solid–liquid phase transfer catalyst in the reduction of aromatic nitro compounds. This silica-grafted polyethylene glycol is proved to be an efficient heterogeneous catalyst in the reduction of nitroarenes to the corresponding aromatic amines with zinc powder in water. The reduction reactions proceeded efficiently with exce...

Large volumes of Japanese encephalitis (JE) virus propagated in mouse brain can be easily purified by polyethylene glycol 6,000. By using the polyethylene glycol precipitation method, mouse hemoglobin was almost all separated from the viral suspension, and consequently the total amount of nonviral protein in the viral suspension decreased. The recovery of infectivity was about 100%. The removal...

to determine the effects of priming and planting density on improvement of morphological and physiological traits of the two hybrid corns sc (s.c. 704) under double cropping in sari, a split plot experiment as randomized complete block design with four replications was conducted in 2010. treatments included two densities of 80 and 100 thousand plants per hectare as the first factor and osmoprim...

The formation of microparticles (MPs) biocompatible and biodegradable hydrogels such as polyethylene glycol diacrylate (PEGDA) utilizing microfluidic devices is an attractive option for entrapment encapsulation active principles microorganisms. Our research group has presented in previous studies a formulation to produce these with adequate physical mechanical characteristics their use the MPs....

We describe a rapid, reliable radioimmunoassay for unconjugated estriol in plasma. Polyethylene glycol (Carbowax 6000) is used to separate antibody-bound and free steroid. The assay is sensitive (25 pg for standards), precise, and accurate. At high and low concentrations of estriol, intra-assay coefficients of variation were 7.1% and 7.6%, respectively, and inter-assay coefficients of variation...

In this work, a systematic study of the viscoelastic properties of hydrogels based on polyethylene glycol diacrylate (PEG-DA) is presented. In addition to artificial PEG-DA-based hydrogels, natural hydrogels in the form of human articular cartilage were examined. Specimens were (unconfined) compression tested under static and dynamic load. Besides this, instrumented indentation tests with diffe...

A widespread application of 3D bioprinting in basic and translational research requires accessibility to affordable printers able produce physiologically relevant tissue models. To facilitate the use as a standard technique biology, an open-source device based on consumer-grade stereolithography apparatus (SLA) printer is developed. This SLA bioprinter can complex constructs that preserve cell ...