background :polymerase chain reaction (pcr) assay has widely used for the detection of tuberculosis (tb). this study tried to compare in-house pcr with some well-known commercial pcr kits for detection of tb agent. methods : clinical samples obtained from 620 tb suspected patients were analyzed for the diagnosis of mycobacterium tuberculosis complex (mtc) by in-house pcr. all samples were obtai...

background toxoplasmosis is a parasitic disease caused by the protozoan toxoplasma gondii. this parasite infects most of warm-blooded animals, including birds. turkeys are one of these animals which might be infected by this parasite. little is known about the prevalence of t. gondii in turkeys in iran. objectives the current study aimed to evaluate the rate of toxoplasma infection in turkeys i...

background: acute infectious diarrhea is one of the most important causes of morbidity and mortality worldwide. objectives: the objective of this study was to characterize antimicrobial resistant diarrheagenic escherichia coli strains isolated from diarrheic children in tehran, ir iran. patients and methods: in total, 550 stool samples from diarrheic pediatric patients, aged less than 60 months...

background: human papillomaviruses (hpvs) are the most common viruses which can be sexually transmitted. they can cause different malignancies in asymptomatic women. the association of hpvs with infertility among men and women is controversial. in the current study, the authors compared the frequency of hpvs in fertile and infertile women in the city of mashhad. materials and methods: in the pr...

results of 50 patients, 20 were men and 30 women with mean age of 61.1 ± 16.9 years ranged between 22 and 85 years. all samples of pterygia had positive results for adenoviruses dna with polymerase chain reaction, but none of the negative control groups displayed adenoviruses. the pterygium group and the control groups were β-globin positive. direct sequencing of pcr products confirmed adenovir...

background aggregatibacter actinomycetemcomitans and tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. the detection of these bacteria is needed for diagnosis and management of the mentioned diseases. objectives we aimed to develop and compare improved multiplex conventional and sybr green real time pcr assays for a specific diagnosis of the organisms...

conclusions: vegetables are a new source of h. pylori and accurate washing of vegetables improves such contaminations. results: the results showed that 44 of 460 samples (9.56%) were positive for h. pylori using the culture method. the polymerase chain reaction technique showed that 50 of 460 samples (10.86%) were positive for h. pylori. un-washed leek, traditional salad, un-washed basil and un...

abstract background brucellosis is endemic in iran, and is seen in all age groups, including children. its diagnosis in childhood needs a high index of suspicion. the diagnostic methods currently in use need evaluation for analytical performance. objective the present study tries to evaluate the hematological indices, serologic markers of inflammation and symptoms in patients suspected of bruce...

background: colorectal tumor is one of the main causes of death in our country. the aim of the present study was to determine the clinicopathological aspects of tumor and the presence of hepatic micrometastasis in patients with colorectal cancer (crc). materials and methods: forty two patients with crc were evaluated in the study surgical treatment was performed and liver biopsy was taken for t...