In an earlier paper (Y. Yamamura et al., Am. Rev. Respirat. Diseases 91:839, 1965; I. Azuma et al., Am. Rev. Respirat. Diseases, in press), we reported the isolation and purification of a polysaccharide from a culture filtrate of human tubercle bacilli Aoyama B strain. The polysaccharide was shown to have anaphylactic activity in guinea pigs which were sensitized by heatkilled tubercle bacilli....

Separation of the wound exudate from Acacia senegal (L.) Willd., "gum arabic," on a preparative Superose-6 column gave two major fractions: a high molecular weight gum arabic glycoprotein (GAGP) containing about 90% carbohydrate and a lower molecular weight heterogenous gum arabic polysaccharide fraction. Hydrogen fluoride-deglycosylation of GAGP gave a large ( approximately 400 residue) hydrox...

An analysis of which component of lipopolysaccharide, the lipid or the polysaccharide, is mitogenic for mouse B-lymphocytes has been performed. A purified glycolipid derived from a rough mutant of Salmonella minnesota (R595) that does not contain any o-polysaccharide at all is more mitogenic than an intact lipopolysaccharide derived from a smooth strain of S. minnesota. Results using fractions ...

May, Lewis K. (Woman's Medical College of Pennsylvania, Philadelphia), Ralph A. Knight, and H. William Harris. Allescheria boydii and Aspergillus fumigatus skin test antigens. J. Bacteriol. 91:2155-2157. 1966.-Protein and polysaccharide fractions were extracted from culture filtrates of Allescheria boydii and Aspergillus fumigatus by the methods of Seibert and of Heidelberger, and injected intr...

Plant primary cell walls are composite structures surrounding the protoplast and containing pectins, hemicelluloses, cellulose polysaccharides, as well proteins. Their composition changed during evolution of green lineage from algae to terrestrial plants, i.e., an aquatic a environment. The constraints life in environments have generated new requirements for organisms, necessitating adaptations...

The cell-associated dextransucrase produced by sucrose-grown cells of cariogenic Streptococcus mutans K1-R is derived from soluble dextransucrase. Synthesis of insoluble dextran by soluble dextransucrase gives rise to two dextransucrase fractions bound to the insoluble polysaccharide; a reversibly bound enzyme, which can be eluted in solutions of clinical dextran, and an irreversibly bound enzy...

Aconitum kusnezoffii Reichb., one of the earliest recorded toxic species of genus Aconitum, has been used as traditional Chinese medicine and medicinal diet over the last 2500 years to treat heart failure congestion, neuralgia, rheumatism and gout, etc. In the present paper, four water-soluble polysaccharide fractions isolated from the tubers of A. kusnezoffii Reichb. were studied the antioxida...

The effects of purified non-starch polysaccharide (NSP) fractions (soluble, guar gum (SOL) and insoluble, cellulose (INSOL)) NSP fractions on digesta passage rate, retention time and voluntary feed intake was studied in grower pigs. Compared to control, adding SOL+INSOL increased digesta viscosity, slowed digesta flow rate and increased retention time in the total tract. Voluntary feed intake a...

1. A considerable advance has been made in the study of the fulvic extract of soil organic matter. A new method has been introduced whereby the fulvic extract is separated by a selective adsorption technique into four distinct fractions. 2. These fractions have been obtained from the fulvic extracts from four widely diverse types of soil. 3. A preliminary examination of the chemical properties ...