The plant viral family Luteoviridae is divided into three genera: Luteovirus, Polerovirus and Enamovirus. Without assistance from another virus, members of the family are confined to the cells of the host plant's vascular system. The first open reading frame (ORF) of poleroviruses and enamoviruses encodes P0 proteins which act as silencing suppressor proteins (VSRs) against the plant's viral de...

Abstract Five grapevine viruses, including geminivirus A (GGVA), Syrah virus 1 (GSyV-1), Cabernet Sauvignon reovirus (GCSV), F (GVF) and Red Globe (GRGV) have been detected in four South Australian vineyards using metagenomic high throughput sequencing for the first time Australia. Most of them were present asymptomatic grapevines some with leafroll disease that infected by several other leafro...

Tepfer, M. 1993. Viral genes and transgenic plants. What are potential environmental risks? Bio/Technology 11:1125–1132. Truve, E., A. Aaspollu, J. Honkanen, R. Puska, M. Mehto, A. Hassi, T.H. Teeri, M. Kelve, P. Seppanen, and M. Saarma. 1993. Transgenic potato plants expressing mammalian 2'-5' oligoadenylate synthetase are protected from potato virus X infection under field conditions. Bio/Tec...

A full-length cDNA corresponding to the RNA genome of Potato leafroll virus (PLRV) was modified by inserting cDNA that encoded the jellyfish green fluorescent protein (GFP) into the P5 gene near its 3' end. Nicotiana benthamiana protoplasts electroporated with plasmid DNA containing this cDNA behind the 35S RNA promoter of Cauliflower mosaic virus became infected with the recombinant virus (PLR...

Northern blot analysis of nucleic acid from potato plant tissues and tobacco protoplasts infected with a Scottish isolate of potato leafroll luteovirus (PLRV) detected the 6 kb genomic RNA and one subgenomic RNA species of about 2.7 kb; RNA extracted from virus particles contained only the genomic species. Blotting with small defined probes suggested that the location of the 5' end of the subge...