Genomic selection has transformed the livestock industry, enabling early-life of animals. Biopsy sampling pre-implantation embryos been described since 1968. However, it was only after 2010, with advancement molecular biology techniques such as whole genomic amplification and SNP Chips, that next-generation sequencing became commercially available for bovine embryos. It is now possible to make ...

Pig cloning by somatic cell nuclear transfer (SCNT) remains extremely inefficient, and many cloned embryos undergo abnormal development. Here, by profiling transcriptome expression, we observed dysregulated chromosome-wide gene expression in every chromosome and identified a considerable number of genes that are aberrantly expressed in the abnormal cloned embryos. In particular, XIST, a long no...

Rhein, a glucoside chemical compound found in a traditional Chinese medicine derived from the roots of rhubarb, induces cell apoptosis and is considered to have high potential as an antitumor drug. Several previous studies showed that rhein can inhibit cell proliferation and trigger mitochondria-related or endoplasmic reticulum (ER) stress-dependent apoptotic processes. However, the side effect...

Different livestock sectors as beef industries get benefit from the production of male animals while dairy industries get benefit from the milk production by the female animals. Therefore, it is obvious need to produce the animals of desired sex which can be achieved by predetermining the sex of conceptus at the time of conception i.e. pre determination of sex may be of great economic importanc...

background: the mitochondria are an important source of adenosine triphosphate (atp) production in pre-implantation embryo. therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and atp content. methods: the embryos at 2-pn, 4-cell and blastocyst stages were collected from the oviduct of st...

The hormone leptin, which is primarily produced by adipose tissue, is a critical permissive factor for multiple reproductive events in the mouse, including implantation. In the CD1 strain, maternally derived leptin from the oocyte becomes differentially distributed among the blastomeres of pre-implantation embryos to create a polarized pattern, a feature consistent with a model of development i...

The integrity of male germ cell genome is critical for the correct progression of spermatogenesis, successful fertilization, and proper development of the offspring. Several DNA repair pathways exist in male germ cells. However, unlike somatic cells, key components of such pathways remain largely unidentified. Gametogenetin (GGN) is a testis-enriched protein that has been shown to bind to the D...

introduction: this investigation was undertaken to examine the effect of 4centigrade degree temperature on development and implantation rate of mouse embryos. materials and methods: femal mice (4-6 week old) were induced to superovulate and were mated. two cell embryos were flushed from excited oviducts, 46 – 48 h after the infection of hcg, and were cultured to eight – cell stage in m16 medium...

Title of Document: EVALUATION OF LEF1 TRANSCRIPTION FACTOR IN MAMMALIAN PREIMPLANTAITON EMBRYOS Ashley Elaine Meece, Master of Science, 2007 Directed By: Associate Professor, Dr. Carol L. Keefer Department of Animal and Avian Sciences The first lineage differentiation of cells during pre-implantation embryo development is critical for continued embryonic growth. Though several transcription fac...