Correlation and validation of the results simulated gastrointestinal digestion food compounds towards in vivo data is essential. The objective this work was to monitor milk micellar casein porcine upper intestinal tract match outcome with gastric vitro following Infogest harmonized protocol. In pig duodenum, small amounts intact caseins were present all samples, while observed up 60 min digesti...

Wiskott Aldrich Syndrome protein (WASP) has a unique regulatory role in sealing ring formation and bone resorption in osteoclasts. Here, using the TAT-transduction method, we show the possible role of WASP domain(s) in sealing ring formation and bone resorption. Transduction of TAT-fused full-length WASP peptide induced Arp2/3 complex formation, F-actin content, sealing ring formation and bone ...

Treatment of rats with the beta-agonist isoproterenol causes a dramatic increase in a series of proteins rich in proline (proline-rich proteins) in the parotid glands (Muenzer, J., Bildstein, C., Gleason, M., and Carlson, D. M. (1979) J. Biol. Chem. 254, 5623, 5629). These proteins which contain about 43% proline, comprise more than 50% of the total soluble protein of glands of rats treated wit...

When subjected to thiol reduction, purified intestinal mucins have been shown to undergo a decrease in molecular mass and to liberate a 118-kDa glycopeptide (Roberton, A. M., Mantle, M., Fahim, R. E. F., Specian, R., Bennick, A., Kawagishi, S., Sherman, P., and Forstner, J. F. (1989) Biochem. J. 261, 637-647). The latter has been called a putative "link" component because it is assumed to be im...

The angiogenesis inhibitor histidine-rich glycoprotein (HRG) constitutes one of several examples of molecules regulating both angiogenesis and hemostasis. The antiangiogenic properties of HRG are mediated via its proteolytically released histidine- and proline-rich (His/Pro-rich) domain. Using a combination of immunohistochemistry and mass spectrometry, we here provide biochemical evidence for ...

background: alzheimer’s disease (ad) is one of the most important neurodegenerative disorder. anti‑cyclic citrullinated peptide (anti‑ccp) may all be involved in the development of vascular disease such as ad. the aim of this study is detection of seropositivity for anti‑ccp antibody in ad patients. methods: in our study, 30 patients with ad and 29 healthy controls (age and‑sex matched) were re...

In humans, male and female partners contribute more or less equally to the infertility problem. In approximately 20% of infertile couples, the concurrence of male and female factors is suggested to be responsible for infertility. Neither of these factors are known nor is there a model system to prove this assumption. We present such a model system in the mouse, in which the lack of acrosin in t...

Sodium dodecyl sulfate-acrylamide gel electrophoresis and molecular-sieve chromatography on 8% agarose demonstrate the existence of a very high molecular weight (500,000-600,000), proline-rich protein in cultured 3T6 fibroblasts that appears to be the precursor molecule (procollagen) of collagen. The kinetics of [(3)H]-proline uptake indicate that this precursor is synthesized at a different ra...

Submit Manuscript | http://medcraveonline.com Abbreviations: AA: Amino Acid; BAP: Bioactive Peptide; BBB: Blood Brain Barrier; BCAA: Branched Chain Amino Acid; BMI: Body Mass Index; CCK: Cholecystokinin; CHO: Carbohydrate; DPP-IV: Dipeptidyl Peptidase IV; EWH: Egg White Hydrolysate; GI: Gastro-Intestinal Tract; GLP-1: Glucagon like Peptide; GMP: Glycomacropeptide; ICV: Intracerebroventricular; ...