Aspergillus niger is a highly potent fungus used in the production of alkaline protease. Extra cellular alkaline protease was purified from A. niger in a twostep procedure involving ammonium sulphate precipitation and Sephadex G100 column chromatography. The molecular mass of the enzyme was determined to be 60 kDa by SDS-PAGE. The enzyme activity was also analyzed by zymogram with gelatin. The ...

In the present study, Bacillus subtilis AP-CMST W3 was isolated from an estuarine environment for the production of protease for various biotechnological applications. The process parameters were optimized to enhance the production of protease by the candidate organism. Protease production was found to be optimum in the presence of 0.5% maltose, 0.5% casein, 0.1% magnesium chloride, 3% sodium c...

Protease executes a large variety of functions and it has many biotechnological applications. It represents as one of the third largest group of industrial enzyme and finds its applications in detergents, leather industry, pharmaceutical industry etc. Among the different vegetable waste products, peel of Manihot esculenta (Tapioca/ cassava) was used as substrates for the production of protease ...

Natural senescence is associated with several fold increase in activity of protease and is characterized by the degradation and loss of proteins therefore plant proteases have been a common studied interestingly. Protease from senesced leaves of the papaya plant (Carica Papaya) was partially purified by ammonium sulfate precipitation. It showed 15 fold purification. Optimum protease activity wa...

1. Fibrinolysin-activated lysin factor and chloroform-activated serum protease of serum and plasma are one and the same enzyme, differing only in their mode of activation. 2. The enzyme as it normally occurs in serum or plasma is not inactive because of combination with serum inhibitor. It is present as an inactive precursor or zymogen and may be activated from this state by streptococcal fibri...

Animal fleshing, the major proteinaceous solid waste generated from leather industry, was used as substrate for the production of alkaline protease by Bacillus cereus 1173900 in solid-state fermentation (SSF). Maximum protease activity of 12,310 U/g was observed at 60 h in SSF crude extract. The extracted protease enzyme was purified by 53.64-fold through ammonium sulphate precipitation and chr...

Studies were conducted on the Bacillus sp. neutral protease immobilization on different matrices. The immobilized protease was prepared by three methods, such as entrapment on calcium alginate and gelatin; physical adsorption by celite and alumina; ionic binding by amberlite IR-120 and DEAE-cellulose. The maximum immobilization yield was obtained with celite adsorbed protease. The immobilized e...

Enzymatic catalysis of biochemical reactions is essential to all living systems. The "lock and key" and "induced fit" models were early contributions to our understanding of the mechanisms involved in the reaction between an enzyme and its substrate. However, whether a given substrate-induced conformation is rigid or remains flexible has not yet been determined. By measuring the enzyme activity...