A genomic clone encoding the pathogenesis-related (PR) lb protein was cloned from Nicotiana tabacum cv. Samsun NN genomic library screening with cDNA clone of PRla protein gene (1). The following nucleotide sequence contains coding and its flanking regions. The nucleotide sequence of PRlb protein gene is highly similar to that of PRla (2-5). Coding region is from 457 to 963 without intron and c...

charcot-marie-tooth disease (cmt) is the most common form of inherited peripheral neuropathy. cmt is genetically and clinically heterogenous group of hereditary motor and sensory neuropathies characterized by areflexia, distal sensory loss and progressive weakness of the distal limb muscles. the x-linked cmt (cmtx) is the second most frequent form of charcot-marie-tooth disease. the dominant cm...

The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae) was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is 15,417 bp, consisting of 13 protein-coding, two rRNA, and 21tRNA genes (the trnaM failed to be sequenced) ...

We sequenced the complete mitochondrial genome of the pirarucu, Arapaima gigas, the largest fish of the Amazon basin, and economically one of the most important species of the region. The total length of the Arapaima gigas mitochondrial genome is 16,433 bp. The mitochondrial genome contains 13 protein-coding genes, two rRNA genes and 22 tRNA genes. Twelve of the thirteen protein-coding genes ar...

We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association with the coding region is shown to be maintained, even during very intensive tr...

The sequence of human foetal liver aldose reductase cDNA obtained from a \-gtlO library 1s presented. This cDNA encodes a protein of 316 ami no adds with a 45 bp 5'-non coding region and a 356 bp 3 1 non coding region. Aldose reductase Is of wide clinical Interest 1n that Inhibitors may prevent the complications associated with diabetes melHtus. The cDNA clone was Identified using ol1gonucleot1...

DNA/RNA Description TRPV1 gene consists of 17 exons and 17 introns including a coding region and a 5' and a 3' non-coding region. Transcription There are four transcript variants encoding the same protein, but with different segments in the 5' UTR (var.1, var.2, var.3, var.4) and one alternative splice variant lacking exon 7 (TRPV1b). TRPV1 gene transcription was demonstrated in different cells...

The prion protein (PrP) plays a central role in the pathogenesis of Creutzfeldt-Jakob disease and other transmissible spongiform encephalopathies (TSEs). Mutations in the coding region of the prion protein (PRNP) gene are linked to inherited forms of TSEs whereas aetiology of sporadic CJD (sCJD) remains obscure. It remains unclear whether the primary DNA sequence at non-coding region of PRNP ge...

The problem of predicting gene locations in newly sequenced DNA is well known but still far from being successfully resolved. A novel approach to the problem based on the frame dependent (non-homogeneous) Markov chain models of protein-coding regions was previously suggested. This approach is, apparently, one of the most powerful "search by content" methods. The initial idea of the method combi...

The half-life of c- myc mRNA is regulated when cells change their growth rates or differentiate. Two regions within c- myc mRNA determine its short half-life. One is in the 3'-untranslated region, the other is in the coding region. A cytoplasmic protein, the coding region determinant-binding protein (CRD-BP), binds in vitro to the c- myc coding region instability determinant. We have proposed t...