Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...

Aldosterone, the major sodium retaining hormone in man, will stimulate active transport of sodium across the urinary bladder of the toad, Bufo marinus in vitro, at physiological concentrations of the hormone. The in vitro action of aldosterone is mimicked by steroid hormones with known mineralocorticoid proper­ ties and it is competitively inhibited by other analogues, e.g. spironolactone and c...

The impact of PGF2? on total proteins, free amino acids and the amino transferases in the gonads of silkworm, Bombyx mori L. were investigated in the present experiment. The effect of PGF2? resulted in increase in the total protein and free amino acid contents in the gonads of silkworm larvae indicating improved structural and dynamic levels of organization. The enhanced activity levels of amin...

the aim of this study was to establish and optimise a simplified method of halved grain culture, in order to utilise it to evaluate the ability of isolated sinks (developing wheat grains) in sucrose uptake and conversion (defined by sink strength) under unlimited source conditions. both sucrose uptake and starch synthesis increased linearly over the incubation periods used in this study. a ...

Intradialytic protein catabolism is attributed to loss of amino acids in the dialysate. We investigated the effect of amino acid infusion during hemodialysis (HD) on muscle protein turnover and amino acid transport kinetics by using stable isotopes of phenylalanine, leucine, and lysine in eight patients with end-stage renal disease (ESRD). Subjects were studied at baseline (pre-HD), 2 h of HD w...

The hemopoietic activity of the human placenta was studied in vitrc under various conditions in order to assess the optimal conditions of protein synthesis and colony stimulating factor (CSF) production by this tissue. The hemopoietic activity of placental tissue was assayed by the semi -solid agar medium technique. In order to obtain maximum hemopoietic activity, various stimulators were ...