نتایج جستجو برای: pxo2
تعداد نتایج: 87 فیلتر نتایج به سال:
Search for Bacillus anthracis potential vaccine candidates by a functional genomic-serologic screen.
Bacillus anthracis proteins that possess antigenic properties and are able to evoke an immune response were identified by a reductive genomic-serologic screen of a set of in silico-preselected open reading frames (ORFs). The screen included in vitro expression of the selected ORFs by coupled transcription and translation of linear PCR-generated DNA fragments, followed by immunoprecipitation wit...
Genome engineering is a powerful method for the study of bacterial virulence. With the availability of the complete genomic sequence of Bacillus anthracis, it is now possible to inactivate or delete selected genes of interest. However, many current methods for disrupting or deleting more than one gene require use of multiple antibiotic resistance determinants. In this report we used an approach...
BACKGROUND Inhalation anthrax is characterized by a systemic spread of the challenge agent, Bacillus anthracis. It causes severe damage, including multiple hemorrhagic lesions, to host tissues and organs. It is widely believed that anthrax lethal toxin secreted by proliferating bacteria is a major cause of death, however, the pathology of intoxication in experimental animals is drastically diff...
In an ongoing Microbial Observatory investigation of the International Space Station (ISS), 11 Bacillus strains (2 from the Kibo Japanese experimental module, 4 from the U.S. segment, and 5 from the Russian module) were isolated and their whole genomes were sequenced. A comparative analysis of the 16S rRNA gene sequences of these isolates showed the highest similarity (>99%) to the Bacillus ant...
Background: The secretion time course of Bacillus anthracis strain RA3R (pXO1+/pXO2-) during early, mid, and late log phase were investigated under conditions that simulate those encountered in the host. All of the identified proteins were analyzed by different software algorithms to characterize their predicted mode of secretion and cellular localization. In addition, immunogenic proteins were...
doi:10.4102/ojvr.v81i2.722 http://www.ojvr.org Authors: B.M. Hang’ombe1 M. Ziwa2 M. Haule2 I. Nakamura3 K.L. Samui1 D. Kaile4 A.S. Mweene1 B.S. Kilonzo5 E.F. Lyamuya2 M. Matee2 C. Sugimoto3 H. Sawa3 B.W. Wren6 Affiliations: 1School of Veterinary Medicine, University of Zambia, Zambia 2Muhimbili University of Health and Allied Sciences, Tanzania 3Hokkaido University Research Center for Zoonosis ...
Identification of Bacillus anthracis is considerably difficult because of its very high phenotypic and genotypic similarity to other members of the Bacillus cereus group. Differentiation methods based on morphological and phenotypic characteristics are time-consuming, and false results may be obtained for atypical strains. On the other hand molecular discrimination based on the presence of two ...
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