The limit of detection and limit of quantification are current critical issues in environmental testing. In most laboratories, limits are currently calculated on the basis of the standard deviation of replicate analyses at a single concentration. However, since the standard deviation depends on concentration, these single-concentration techniques result in limits that are directly dependent on ...

BACKGROUND To date, over 150 genetically modified (GM) crops are widely cultivated. To comply with regulations developed for genetically modified organisms (GMOs), including labeling policies, many detection methods for GMO identification and quantification have been developed. RESULTS To detect the entrance and exit of unauthorized GM crop events in China, we developed a novel quadruplex rea...

Swertiachirata is an endangered medicinal herb with a wide range of pharmacological activities, particularly as an anti-diabetic in an herbal formulation Diabecon (Himalaya®). The endangered status has resulted in short supply of plant material, thereby, resulting in adulteration practices. It is, therefore, of paramount importance to develop a shoot biomass production system enriched with majo...

a rapid, sensitive and reproducible hplc method using amperometric detector was developed and validated for the analysis of clarithromycin in human plasma. the separation was achieved on a monolithic silica column (mz- c8 125×4.0 mm) using acetonitrile- methanol-potassium dihydrogen phosphate buffer (40:6:54,v/v), with ph of 7.5, as the mobile phase at a flow rate of 1.5 ml/min. the assay enabl...

easily degradating and various isomeric forms of rapamycin (sirolimus) face the determination of this compound to many challenges. in this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (rp-hplc) method for rapamycin. separation was performed on a c8 column (mz, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...

We have developed a novel, single-step, isothermal, signal-amplified, and sequence-specific RNA quantification method (L-assay). The L-assay consists of nicking endonuclease, a dual-labeled fluorescent DNA probe (DL-probe), and conformation-interchangeable oligo-DNA (L-DNA). This signal-amplified assay can quantify target RNA concentration in a sequence-specific manner with a coefficient of var...

We developed and assessed real-time PCR (RTi-PCR) assays for the detection and quantification of the food-borne pathogen Listeria monocytogenes and the closely related nonpathogenic species L. innocua. The target genes were hly and iap for L. monocytogenes and lin02483 for L. innocua. The assays were 100% specific, as determined with 100 Listeria strains and 45 non-Listeria strains, and highly ...

Soot formation in combustors is a complex process comprising highly intermittent interactions between physical and chemical processes across wide range of time-scales the influence turbulence on soot inception remains substantially conjectural. The current study quantifies impact flame temperature, equivalence ratio, strain rate PAH concentrations premixed turbulent ethylene flames crossing lim...