A method for assaying leukocyte pyrogen is described which is shown to remain valid despite the presence of very large amounts of bacterial endotoxin. It uses a combination of two procedures to inactivate endotoxin: incubation in normal rabbit plasma for 24 hr at 37 degrees C, and injection into rabbits rendered refractory to endotoxin. The validity of the assay was confirmed by formal statisti...

One of the major problems in studies on gonadotropin(GTH)is to use the appropriate assay method. While numerous methods of bio-assay for GTH were used by many workers, one of those was based on the weight-increases of gonad and accessory sexual organ of assay animals, and this might be classified into following three groups according to the species of assay animals;rat, mouse and chick method. ...

The contributions to this special issue of Mutagenesis have been selected to cover the main research areas served by the comet assay, namely genotoxicology, environmental toxicology, human biomonitoring and fundamental investigations into mechanisms of DNA damage and repair. Innovative methods are described, technical issues are explored, and guidelines are given for venturing into relatively n...